A Pyrene-Derived Fluorescent Probe for the Visual Detection of Protamine and Trypsin Activity

被引：0

作者：

Xiong, Longjun ^{[1
]}

Jiao, Yutian ^{[1
]}

Liu, Yu ^{[1
]}

Wei, Gongli ^{[1
]}

Xu, Baocai ^{[1
]}

Han, Fu ^{[1
]}

Zhao, Li ^{[1
]}

机构：

[1] Beijing Technol & Business Univ, Sch Light Ind, Beijing 100048, Peoples R China

来源：

JOURNAL OF FLUORESCENCE | 2025年

基金：

国家重点研发计划;

关键词：

Fluorescent probe; Pyrene; Excimer; Protamine; Trypsin; RATIOMETRIC DETECTION; GOLD NANOCLUSTERS; INHIBITOR;

D O I：

10.1007/s10895-024-04122-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A pyrene-derived fluorescent probe (P4CG) was designed and synthesized for the purpose of detecting protamine and trypsin activity. The anionic probe self-assembled with protamine, driven by electrostatic and hydrophobic interactions, exhibiting a sensing behavior towards protamine in a fluorescence ratiometric manner. The assay demonstrated high sensitivity, with a limit of detection (LOD) of 13.8 ng/mL, and exhibited selectivity in the HEPES buffer solution. Moreover, the P4CG-protamine complex enables the monitoring of trypsin activity with satisfactory sensitivity and selectivity. The presence of the trypsin inhibitor resulted in the inhibition of the hydrolysis of protamine, which in turn led to a diminished fluorescence recovery. Consequently, this assay can be employed for the screening of trypsin inhibitors.

引用

页数：8

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