Recent advances in spatiotemporal control of the CRISPR/Cas9 system

被引：4
作者：
Zhang, Junqi [1 ,2 ]
Zhou, Yuzi [1 ]
Qiao, Jie [1 ]
Liu, Yi [2 ]
机构：
[1] Wuhan Polytech Univ, Sch Life Sci & Technol, Wuhan 430023, Hubei, Peoples R China
[2] Hubei Univ, Sch Life Sci, State Key Lab Biocatalysis & Enzyme Engn, Wuhan 430042, Hubei, Peoples R China
来源：
COLLOIDS AND SURFACES B-BIOINTERFACES | 2025年 / 248卷
关键词：
Gene editing; CRISPR/Cas9; Precise regulation and control; Optogenetics; Small molecule regulation; RNA-GUIDED CAS9; PROTEIN INTERACTIONS; DNA INTERROGATION; CHEMICAL CONTROL; CRISPR-CAS9; COMPLEX; ENDONUCLEASE; RIBONUCLEOPROTEIN; SPECIFICITY; INDUCTION;
D O I：
10.1016/j.colsurfb.2024.114474
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
The CRISPR/Cas9 gene-editing technology, derived from the adaptive immune mechanisms of bacteria, has demonstrated remarkable advantages in fields such as gene function research and the treatment of genetic diseases due to its simplicity in design, precise targeting, and ease of use. Despite challenges such as off-target effects and cytotoxicity, effective spatiotemporal control strategies have been achieved for the CRISPR/Cas9 system through precise regulation of Cas9 protein activity as well as engineering of guide RNAs (gRNAs). This review provides a comprehensive analysis of the core components and functional mechanisms underlying the CRISPR/Cas9 system, highlights recent advancements in spatiotemporal control strategies, and discusses future directions for development.
引用
收藏
页数：10
相关论文
共 80 条
[1]   Coupling Cas9 to artificial inhibitory domains enhances CRISPR-Cas9 target specificity [J].
Aschenbrenner, Sabine ;
Kallenberger, Stefan M. ;
Hoffmann, Mareike D. ;
Huck, Adrian ;
Eils, Roland ;
Niopek, Dominik .
SCIENCE ADVANCES, 2020, 6 (06)
[2]   Bridge Helix of Cas9 Modulates Target DNA Cleavage and Mismatch Tolerance [J].
Babu, Kesavan ;
Amrani, Nadia ;
Jiang, Wei ;
Yogesha, S. D. ;
Nguyen, Richard ;
Qin, Peter Z. ;
Rajan, Rakhi .
BIOCHEMISTRY, 2019, 58 (14) :1905-1917
[3]   Chemical control of protein stability and function in living mice [J].
Banaszynski, Laura A. ;
Sellmyer, Mark A. ;
Contag, Christopher H. ;
Wandless, Thomas J. ;
Thorne, Steve H. .
NATURE MEDICINE, 2008, 14 (10) :1123-1127
[4]   A rapid, reversible, and tunable method to regulate protein function in living cells using synthetic small molecules [J].
Banaszynski, Laura A. ;
Chen, Lin-chun ;
Maynard-Smith, Lystranne A. ;
Ooi, A. G. Lisa ;
Wandless, Thomas J. .
CELL, 2006, 126 (05) :995-1004
[5]   Photoisomerization in different classes of azobenzene [J].
Bandara, H. M. Dhammika ;
Burdette, Shawn C. .
CHEMICAL SOCIETY REVIEWS, 2012, 41 (05) :1809-1825
[6]   Guide RNA Functional Modules Direct Cas9 Activity and Orthogonality [J].
Briner, Alexandra E. ;
Donohoue, Paul D. ;
Gomaa, Ahmed A. ;
Selle, Kurt ;
Slorach, Euan M. ;
Nye, Christopher H. ;
Haurwitz, Rachel E. ;
Beisel, Chase L. ;
May, Andrew P. ;
Barrangou, Rodolphe .
MOLECULAR CELL, 2014, 56 (02) :333-339
[7]   CRISPRoff enables spatio-temporal control of CRISPR editing [J].
Carlson-Stevermer, Jared ;
Kelso, Reed ;
Kadina, Anastasia ;
Joshi, Sahil ;
Rossi, Nicholas ;
Walker, John ;
Stoner, Rich ;
Maures, Travis .
NATURE COMMUNICATIONS, 2020, 11 (01)
[8]   Near-infrared optogenetic engineering of photothermal nanoCRISPR for programmable genome editing [J].
Chen, Xiaohong ;
Chen, Yuxuan ;
Xin, Huhu ;
Wan, Tao ;
Ping, Yuan .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2020, 117 (05) :2395-2405
[9]   Analysis of off-target effects of CRISPR/Cas-derived RNA-guided endonucleases and nickases [J].
Cho, Seung Woo ;
Kim, Sojung ;
Kim, Yongsub ;
Kweon, Jiyeon ;
Kim, Heon Seok ;
Bae, Sangsu ;
Kim, Jin-Soo .
GENOME RESEARCH, 2014, 24 (01) :132-141
[10]   Multiplex Genome Engineering Using CRISPR/Cas Systems [J].
Cong, Le ;
Ran, F. Ann ;
Cox, David ;
Lin, Shuailiang ;
Barretto, Robert ;
Habib, Naomi ;
Hsu, Patrick D. ;
Wu, Xuebing ;
Jiang, Wenyan ;
Marraffini, Luciano A. ;
Zhang, Feng .
SCIENCE, 2013, 339 (6121) :819-823
12345678