3D-printing of dipyridamole/thermoplastic polyurethane materials for bone regeneration

被引:1
|
作者
Adhami, Masoud [1 ]
Dastidar, Anushree Ghosh [2 ]
Anjani, Qonita Kurnia [1 ]
Detamornrat, Usanee [1 ]
Tarres, Quim [3 ]
Delgado-Aguilar, Marc [3 ]
Acheson, Jonathan G. [4 ]
Manda, Krishnagoud [2 ]
Clarke, Susan A. [5 ]
Moreno-Castellanos, Natalia [6 ]
Larraneta, Eneko [1 ]
Dominguez-Robles, Juan [1 ,7 ]
机构
[1] Queens Univ Belfast, Sch Pharm, Lisburn Rd 97, Belfast BT9 7BL, North Ireland
[2] Queens Univ Belfast, Sch Mech & Aerosp Engn, Belfast, North Ireland
[3] Univ Girona, Dept Chem Engn, Grp LEPAMAP PRODIS, C-M Aurelia Campmany 61, Girona 17003, Spain
[4] Ulster Univ, Nanotechnol & Integrated Bioengn Ctr NIBEC, Sch Engn, Belfast, North Ireland
[5] Queens Univ Belfast, Sch Nursing & Midwifery, Belfast BT9 7BL, North Ireland
[6] Univ Ind Santander, CICTA, Med Sch, Dept Basic Sci,Hlth Fac, Cra 27 Calle 9, Bucaramanga 680002, Colombia
[7] Univ Seville, Fac Pharm, Dept Pharm & Pharmaceut Technol, Seville 41012, Spain
基金
英国工程与自然科学研究理事会;
关键词
Thermoplastic polyurethane; Fused deposition modelling; 3D printing; Bone regeneration; Dipyridamole; Flexible materials; SCAFFOLDS; RELEASE; DIFFERENTIATION; IMPLANTATION;
D O I
10.1007/s13346-024-01744-1
中图分类号
TH7 [仪器、仪表];
学科分类号
0804 ; 080401 ; 081102 ;
摘要
Tissue engineering combines biology and engineering to develop constructs for repairing or replacing damaged tissues. Over the last few years, this field has seen significant advancements, particularly in bone tissue engineering. 3D printing has revolutionised this field, allowing the fabrication of patient- or defect-specific scaffolds to enhance bone regeneration, thus providing a personalised approach that offers unique control over the shape, size, and structure of 3D-printed constructs. Accordingly, thermoplastic polyurethane (TPU)-based 3D-printed scaffolds loaded with dipyridamole (DIP) were manufactured to evaluate their in vitro osteogenic capacity. The fabricated DIP-loaded TPU-based scaffolds were fully characterised, and their physical and mechanical properties analysed. Moreover, the DIP release profile, the biocompatibility of scaffolds with murine calvaria-derived pre-osteoblastic cells, and the intracellular alkaline phosphatase (ALP) assay to verify osteogenic ability were evaluated. The results suggested that these materials offered an attractive option for preparing bone scaffolds due to their mechanical properties. Indeed, the addition of DIP in concentrations up to 10% did not influence the compression modulus. Moreover, DIP-loaded scaffolds containing the highest DIP cargo (10% w/w) were able to provide sustained drug release for up to 30 days. Furthermore, cell viability, proliferation, and osteogenesis of MC3T3-E1 cells were significantly increased with the highest DIP cargo (10% w/w) compared to the control samples. These promising results suggest that DIP-loaded TPU-based scaffolds may enhance bone regeneration. Combined with the flexibility of 3D printing, this approach has the potential to enable the creation of customized scaffolds tailored to patients' needs at the point of care in the future.
引用
收藏
页数:16
相关论文
共 50 条
  • [21] Soft 3D printing of thermoplastic polyurethane: Preliminary study
    Fenollosa-Artes, Felip
    Jorand, Leo
    Tejo-Otero, Aitor
    Lustig-Gainza, Pamela
    Romero-Sabat, Guillem
    Medel, Sandra
    Uceda, Roger
    PROCEEDINGS OF THE INSTITUTION OF MECHANICAL ENGINEERS PART B-JOURNAL OF ENGINEERING MANUFACTURE, 2023, 237 (6-7) : 1128 - 1135
  • [22] 3D-printing wood
    O'Driscoll, Cath
    CHEMISTRY & INDUSTRY, 2022, 86 (09) : 16 - 16
  • [23] Magnetic annealing of extruded thermoplastic magnetic elastomers for 3D-Printing via FDM
    Fischer, Nathan A.
    Robinson, Alex L.
    Lee, Thomas J.
    Calascione, Thomas M.
    Koerner, Lucas
    Nelson-Cheeseman, Brittany B.
    JOURNAL OF MAGNETISM AND MAGNETIC MATERIALS, 2022, 553
  • [24] Magnetic annealing of extruded thermoplastic magnetic elastomers for 3D-Printing via FDM
    Fischer, Nathan A.
    Robinson, Alex L.
    Lee, Thomas J.
    Calascione, Thomas M.
    Koerner, Lucas
    Nelson-Cheeseman, Brittany B.
    Journal of Magnetism and Magnetic Materials, 2022, 553
  • [25] Using 3D-Printing to Evaluate Trabecular Bone Mechanical Properties
    Barak, Meir M.
    Black, Arielle M.
    FASEB JOURNAL, 2017, 31
  • [26] 3D-printing of Stem Cells and Stromal Cells to Prime for Vascularization and Regeneration
    Peirce-Cottler, Shayn
    FASEB JOURNAL, 2020, 34
  • [27] The application of 3D-printing technology in pelvic bone tumor surgery*
    Park, Jong Woong
    Kang, Hyun Guy
    Kim, June Hyuk
    Kim, Han-Soo
    JOURNAL OF ORTHOPAEDIC SCIENCE, 2021, 26 (02) : 276 - 283
  • [28] 3D-PRINTING OF GELATIN BASED SCAFFOLDS FOR TISSUE REGENERATION: PROCESSING AND CHARACTERIZATION
    Uskokovic, Petar
    Jovanovic, Marija
    Petrovic, Milos
    Stojanovic, Dusica
    Radojevic, Vesna
    TISSUE ENGINEERING PART A, 2022, 28 : S387 - S388
  • [29] Materials and scaffolds in medical 3D printing and bioprinting in the context of bone regeneration
    Heller, M.
    Bauer, H. -K.
    Goetze, E.
    Gielisch, M.
    Ozbolat, I. T.
    Moncal, K. K.
    Rizk, E.
    Seitz, H.
    Gelinsky, M.
    Schrder, H. C.
    Wang, X. H.
    Mueller, W. E. G.
    Al-Nawas, B.
    INTERNATIONAL JOURNAL OF COMPUTERIZED DENTISTRY, 2016, 19 (04) : 301 - 321
  • [30] 3D-printing of Continuous Fiber: A review of processes, materials and properties
    Arabi, Mostafa Khosroupour
    Kordani, Naser
    POLYMER-PLASTICS TECHNOLOGY AND MATERIALS, 2023, 62 (12): : 1525 - 1559