Electrochemical Impedance-Based Detection of Pancreatic Cancer Biomarker Glypican1 and Mucin1 Using Electric Field-Lysed Extracellular Vesicles for Analysis: A Proof of Concept

Glypican1 and mucin1 antigens are prominent biomarkers for the prognosis and diagnosis of pancreatic cancer. Their presence within the extracellular vesicles (EVs) opens the possibilities for oncology care through the development of minimally invasive biomarker-assisted screening tools. Traditionally, EV antigen quantification relies on ultracentrifugation (UC) and chemical lysis, which are time-consuming, equipment-dependent, and often compromise EV integrity, damaging surface intact biomarkers. This study integrates EV isolation and electric field (EF) lysis into a unified platform. The lysates were then analyzed using an electrochemical impedance spectroscopy (EIS)-based sensor to detect glypican-1 (GPC1) and mucin-1 (MUC1). ELISA confirms the EF lysis of the immobilized EV and shows an increase in the antigen concentration by 2.5 times (compared to the pre-lysed sample). Hence, EF lysis makes the sensor more sensitive than traditional methods. To enhance the electric lysis process, we applied varying voltages of a sinusoidal signal to the screen printed gold electrode (SPGE)-immobilized EVs. The lysate was subsequently used to quantify the GPC1 and MUC1 antigens through EIS. The results indicate that a 50-mV sinusoidal signal is sufficient to effectively lyse EVs, confirmed by western blotting. The nanoparticle tracking analyzer (NTA) results showed the successful isolation of 10(9) EVs from 100 mu L of serum using CD63 antibody. The developed EIS sensor can detect GPC1 and MUC1 with an LOD of 0.053 and 0.033 pg/mL, respectively, from EV lysate, showing minimal nonspecific binding in the negative control. Beyond GPC1 and MUC1, the approach is adaptable for detecting other EV-associated biomarkers, enabling broader applications in early cancer detection and disease monitoring.