Generation of a KDM5D knockout human embryonic stem cell line with CRISPR/Cas9 technology

被引：0
|
作者：
Li, Ouhui [1 ,2 ,3 ,4 ,5 ,6 ]
Zhen, Yuting [1 ,2 ,3 ,4 ,5 ,6 ]
Sun, Chao [3 ,4 ,5 ,6 ]
Ma, Yanlin [3 ,4 ,5 ,6 ]
Li, Qi [3 ,4 ,5 ,6 ]
Wen, Luan [1 ,2 ,3 ,4 ,5 ,6 ]
机构：
[1] Hainan Med Univ, Sch Basic Med & Life Sci, Dept Biochem & Mol Biol, Key Lab Trop Translat Med,Minist Educ, Haikou 571199, Hainan, Peoples R China
[2] Hainan Med Univ, Sch Basic Med & Life Sci, Dept Biochem & Mol Biol, Key Lab Brain Sci Res Transformat Trop Environm Ha, Haikou 571199, Hainan, Peoples R China
[3] Hainan Med Univ, Affiliated Hosp 1, Hainan Prov Key Lab Human Reprod Med & Genet Res, Key Lab Reprod Hlth Dis Res & Translat,Minist Educ, Haikou 571101, Hainan, Peoples R China
[4] Hainan Med Univ, Affiliated Hosp 1, Hainan Prov Clin Res Ctr Thalassemia, Dept Reprod Med, Haikou 571101, Hainan, Peoples R China
[5] Minist Sci & Technol China, Natl Ctr Int Res China Myanmar Joint Res Ctr Preve, Haikou 571101, Hainan, Peoples R China
[6] Hainan Med Univ, Haikou Key Lab Preservat Human Genet Resource, Affiliated Hosp 1, Haikou 571101, Hainan, Peoples R China
来源：
STEM CELL RESEARCH | 2025年 / 83卷
关键词：
D O I：
10.1016/j.scr.2025.103651
中图分类号：
Q813 [细胞工程];
学科分类号：
摘要：
KDM5D is a gene implicated in spermatogenic failure and sex-related differences in colon cancer progression, though its role in spermatogenesis remains unclear. We successfully generated a KDM5D knockout human embryonic stem cells using CRISPR/Cas9 technology. This knockout cell line provides a valuable model for studying KDM5D's function in spermatogenesis and its influence on sex differences in various diseases.
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页数：6
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