Design of a Golden Gate Cloning Plasmid for the Generation of a Chimeric Virus-Like Particle-Based Subunit Vaccine Against Porcine Circovirus Type 2

被引：0

作者：

Hernandez-Avina, Ana del Socorro ^{[1
]}

Mendoza-Gomez, Luis Fernando ^{[1
]}

Hernandez-Mancillas, Xitlalli D. ^{[2
]}

Salazar-Gonzalez, Jorge Alberto ^{[1
]}

Zapata-Cuellar, Lorena ^{[1
]}

Camacho-Ruiz, Rosa Maria ^{[2
]}

Comas-Garcia, Mauricio ^{[3
,4
]}

Sarmiento-Silva, Rosa Elena ^{[5
]}

Rodriguez, Jorge A. ^{[2
]}

Arellano-Plaza, Melchor ^{[2
]}

Flores-Valdez, Mario Alberto ^{[1
]}

Gutierrez-Ortega, Abel ^{[1
]}

机构：

[1] Ctr Invest & Asistencia Tecnol & Diseno Estado Jal, Unidad Biotecnol Med & Farmaceut, Normalistas 800, Colinas De La Normal 44270, Jalisco, Mexico

[2] Ctr Invest & Asistencia Tecnol & Diseno Estado Jal, Unidad Biotecnol Ind, Camino Arenero 1227, El Bajio Del Arenal 45019, Jalisco, Mexico

[3] Univ Autonoma San Luis Potosi, Ctr Invest Ciencias Salud & Biomed, Secc Microscopia Alta Resoluc, Sierra Leona 550,Lomas Segunda Secc, San Luis Potosi 78210, Mexico

[4] Univ Autonoma San Luis Potosi, Fac Ciencias, Parque Chapultepec 1570, San Luis Potosi 78210, Mexico

[5] Univ Nacl Autonoma Mexico, Fac Med Vet & Zootecnia, Dept Microbiol & Inmunol, Univ 3000,Ciudad Univ, Mexico City 04510, Mexico

来源：

MOLECULAR BIOTECHNOLOGY | 2024年

关键词：

Porcine circovirus type 2; Animal health; Immunization; Veterinary vaccines; Virus-like particles; Papaya ringspot virus; PROTECTIVE IMMUNITY; NONPATHOGENIC PCV1; GENOMIC BACKBONE; WASTING SYNDROME; CAPSID GENE; PROTEIN; DNA; SOLUBILIZATION; PURIFICATION; EXPRESSION;

D O I：

10.1007/s12033-024-01334-z

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Porcine circovirus type 2 (PCV2) is a pervasive pathogen in the swine industry, leading to a spectrum of disorders known as porcine circovirus associated diseases (PCVAD). The PCV2 Cap protein contains critical antigenic epitopes and is the primary target for vaccine development. Current vaccines include inactivated viral particles and virus-like particles (VLPs), with experimental vaccines exploring various innovative approaches. This study introduces a novel Golden Gate assembly-based, Escherichia coli expression destination plasmid, pPRSVCP-E18, designed for the in-frame insertion of short peptide-coding DNA sequences into a highly antigenic site of Papaya ringspot virus (PRSV) coat protein (CP). We successfully cloned four PCV2 Cap peptides into this plasmid, expressed the chimeric CP proteins in E. coli under flask and bioreactor conditions, and assembled them into VLPs. These VLPs, when adjuvated and administered to BALB/c mice, elicited a specific IgG response against the PCV2 Cap peptides. Our findings demonstrate the plasmid's high efficiency for Golden Gate cloning and its potential in developing subunit vaccines against PCV2, contributing to the sustainable control of PCVAD in the swine industry.

引用

页数：13

共 45 条

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