Comparative efficacy of HPV 16/18 DNA and E6/E7 mRNA testing in detecting high-grade cervical lesions (CIN2+) in women with cervical biopsies

被引：0

作者：

Benvari, Sepideh ^{[1
]}

Aslanimehr, Masoumeh ^{[2
]}

Samiee-Rad, Fatemeh ^{[3
]}

Naserpour-Farivar, Taghi ^{[2
]}

Sadeghi, Hamid ^{[2
,4
]}

机构：

[1] Univ St Andrews, Sch Med, Infect & Global Hlth Res Div, St Andrews, Scotland

[2] Qazvin Univ Med Sci, Med Microbiol Res Ctr, Qazvin, Iran

[3] Qazvin Univ Med Sci, Inst Prevent Noncommunicable Dis, Cellular & Mol Res Ctr, Qazvin, Iran

[4] Qazvin Univ Med Sci, Student Res Comm, Qazvin, Iran

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2025年 / 111卷 / 03期

关键词：

Cervical cancer; E6/E7; mRNA; HPV; 16/18; Real-Time PCR; CANCER; E6; EXPRESSION; CLASSIFICATION; TRIAGE;

D O I：

10.1016/j.diagmicrobio.2024.116668

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

The study evaluated the efficacy of HPV 16/18 E6/E7 mRNA detection in women with abnormal cervical histology. A total of 99 cervical biopsy samples were analyzed, including 49 benign, 16 with cervical intraepithelial neoplasia grade 1 (CIN1), 9 with CIN2/3, and 25 with cervical cancers. Samples were tested for HPV 16/18 using both DNA and mRNA RT-PCR methods. The findings revealed a sensitivity of 85.3 % (29/34) for the HPV DNA test and 76.5 % (26/34) for the mRNA test in detecting CIN2+ lesions. Notably, the E6/E7 mRNA test demonstrated greater specificity for CIN2+ at 75.4 % (49/65), compared to 52.3 % (34/65) for the DNA test. The prevalence of positive results for both tests increased with the severity of squamous cell abnormalities. However, the HPV 16/18 E6/E7 mRNA test provided superior specificity, making it a more effective method for cervical cancer screening in this region, offering more precise results than DNA testing alone.

引用

页数：5

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