Whispers in the Wind: Face Mask Sampling for Mycobacterium tuberculosis Detection in Children With Pulmonary Tuberculosis

被引：0

作者：

Meiwes, Lennard ^{[1
,2
,3
]}

Kontsevaya, Irina ^{[1
,2
,3
,4
]}

Chesov, Dumitru ^{[1
,5
]}

Kulcitkaia, Stela ^{[5
]}

Dreyer, Viola ^{[6
]}

Hillemann, Doris ^{[7
]}

Dlamini, Qiniso ^{[8
,9
,10
]}

Williams, Caroline ^{[11
,12
]}

Barer, Michael ^{[11
,12
]}

Brinkmann, Folke ^{[13
]}

Krueger, Renate ^{[14
,15
]}

Thee, Stephanie ^{[14
,15
]}

Kay, Alexander ^{[8
,9
,10
]}

Mandalakas, Anna Maria ^{[1
,2
,8
,9
]}

Lange, Christoph ^{[1
,2
,3
,8
,9
]}

机构：

[1] Res Ctr Borstel, Clin Infect Dis, Parkallee 35, D-23845 Borstel, Germany

[2] German Ctr Infect Res, Clin TB Unit, Borstel, Germany

[3] Univ Lubeck, Resp Med & Int Hlth, Lubeck, Germany

[4] Imperial Coll London, Dept Infect Dis, Fac Med, London, England

[5] Nicolae Testemitanu State Univ Med & Pharm, Discipline Pneumol & Allergol, Kishinev, Moldova

[6] Leibniz Lung Ctr, Res Ctr Borstel, Mol & Expt Mycobacteriol Grp, Borstel, Germany

[7] Leibniz Lung Ctr, Res Ctr Borstel, Natl Reference Ctr, Borstel, Germany

[8] Baylor Coll Med, Global TB Program, Houston, TX USA

[9] Texas Childrens Hosp, Houston, TX USA

[10] Baylor Coll Med Childrens Fdn Eswatini, TB Ctr Excellence, Mbabane, Eswatini

[11] Univ Leicester, Dept Infect Immun & Inflammat, Leicester, Leics, England

[12] Univ Hosp Leicester NHS Trust, Dept Clin Microbiol, Leicester, Leics, England

[13] Univ Lubeck, Univ Hosp Schleswig Holstein, Dept Pediat, Lubeck, Germany

[14] Charite Univ Med Berlin, Department Pediat Resp Med, Immunol & Crit Care, Berlin, Germany

[15] Charite Univ Med Berlin, Berlin Inst Hlth, Pediat Pulmonol & Immunol, Berlin, Germany

来源：

JOURNAL OF INFECTIOUS DISEASES | 2024年 / 230卷 / 06期

基金：

美国国家卫生研究院;

关键词：

tuberculosis; face masks; PCR; children; diagnosis;

D O I：

暂无

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Background. Recently, face mask sampling (FMS) confirmed detection of Mycobacterium tuberculosis DNA from exhaled breath in adults with tuberculosis. To date, no study has evaluated the use of FMS to detect pulmonary tuberculosis in children. We developed a method for FMS of M. tuberculosis-specific DNA in children and performed a clinical exploration to assess feasibility in children. Methods. Face masks were spiked, analyzed on GeneXpert-Ultra, quantitative polymerase chain reaction, and targeted next-generation sequencing. Children with pulmonary tuberculosis were asked to wear 3 modified FFP2 masks for 30 minutes as part of an exploratory clinical study. Results. Experiments with H37Ra M. tuberculosis strain showed a limit of 95% detection of 3.75 colony-forming units (95% confidence interval, 4.85-3.11) on GeneXpert-Ultra. Ten children with pulmonary tuberculosis participated in the clinical study. M. tuberculosis-specific DNA was detected on none of the face masks. Conclusions. Pediatric FMS has a low limit of detection for M. tuberculosis-specific DNA in vitro. However, M. tuberculosis DNA was not detected in any of 30 masks worn by children with pulmonary tuberculosis. This suggests that FMS in this form may not be more effective for detecting M. tuberculosis in children with tuberculosis than existing methods.

引用

页码：1510 / 1517

页数：8

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