Functional Characterization of the PoWHY1 Gene from Platycladus orientalis and Its Role in Abiotic Stress Tolerance in Transgenic Arabidopsis thaliana

The frequent occurrence of extreme weather conditions in the world has brought many unfavorable factors to plant growth, causing the growth and development of plants to be hindered and even leading to plant death, with abiotic stress hindering the growth and metabolism of plants due to severe uncontrollability. The WHY1 transcription factor plays a critical role in regulating gene expression in plants, influencing chlorophyll biosynthesis, plant growth, and development, as well as responses to environmental stresses. The important role of the PoWHY1 gene in regulating plant growth and adaptation to environmental stress has become a hot research topic. However, the mechanism of the PoWHY1 gene in Platycladus orientalis under abiotic stress is still unclear. Here, the PoWHY1 gene was analyzed bioinformatically using P. orientalis as study material, and the role of the gene against abiotic stress conditions in Arabidopsis thaliana was verified using transgenic technology. It was found that overexpression of PoWHY1 increased seed germination, decreased malondialdehyde accumulation, increased proline content, and delayed the senescence process under salt stress. The expression levels of JAZ1, LOX1, ABI1, and ABI2 were decreased, while the expression levels of RAB18, APX1, GSTF6, and DREB2A were increased, indicating that overexpression of PoWHY1 enhanced the salt stress tolerance of A. thaliana. Furthermore, PoWHY1 overexpression also increased drought tolerance in A. thaliana. From the above results, it can be concluded that maintaining high PoWHY1 expression levels in the leaves of P. orientalis can improve their environmental adaptability. The results provide a scientific basis for understanding the gene function of the PoWHY1 gene of P. orientalis under stress conditions and lay the foundation for further research on the function of the PoWHY1 gene.