Protocol for the visualization of pRps6-positive cells in larval zebrafish brains using whole-mount immunofluorescence and light-sheet microscopy

被引：0

作者：

Doszyn, Olga ^{[1
,2
]}

Dulski, Tomasz ^{[1
]}

Zmorzynska, Justyna ^{[1
,2
]}

机构：

[1] Int Inst Mol Mech & Machines, Lab Dev Neurobiol, PL-02247 Warsaw, Poland

[2] Int Inst Mol & Cell Biol Warsaw, Lab Mol & Cellular Neurobiol, PL-02109 Warsaw, Poland

来源：

STAR PROTOCOLS | 2025年 / 6卷 / 01期

关键词：

developmental biology; microscopy; model organisms; neuroscience;

D O I：

10.1016/j.xpro.2024.103587

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Due to their small size and transparency, larval zebrafish are a useful model for whole-brain imaging. Here, we present a protocol for the visualization of phosphorylated Rps6, a marker of mechanistic target of rapamycin complex 1 (mTORC1) activity, in the zebrafish brains at 5 days post fertilization (dpf), using whole-mount immunofluorescence and light-sheet microscopy. We describe steps for sample preparation, storage, staining, and imaging. This protocol can also be modified for staining with antibodies against other proteins. For complete details on the use and execution of this protocol, please refer to Doszyn et al.1

引用

页数：11

共 5 条

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