An injectable reactive oxygen species-responsive self-assembled hydrogel loaded with L-glutamine targets cartilage repair for treatment of osteoarthritis

被引:0
|
作者
Wang, Chen [1 ,2 ]
He, Junyan [1 ,2 ]
Hu, Zhongyao [1 ,2 ]
Yan, Yiqun [1 ,2 ]
Xu, Zelin [1 ,2 ]
Yu, Yangmang [1 ,2 ]
Su, Jingwen [3 ]
Ma, Bing [4 ]
Yu, Haoran [1 ,2 ]
Zhang, Guiyang [5 ]
Cheng, Wendan [1 ,2 ]
机构
[1] Anhui Med Univ, Dept Orthopaed, Affiliated Hosp 2, 678 Furong Rd, Hefei 230601, Anhui, Peoples R China
[2] Anhui Med Univ, Inst Orthopaed, Res Ctr Translat Med, Affiliated Hosp 2, Hefei 230601, Peoples R China
[3] Anhui Med Univ, Clin Med Coll, Hefei 230031, Peoples R China
[4] Third Peoples Hosp Bengbu, Dept Orthopaed, Bengbu 23300, Peoples R China
[5] Anhui Med Univ, Sch Basic Med Sci, Dept Pharmacol, Hefei 230032, Peoples R China
关键词
Cartilage; Chitosan; Glutamine; Hydrogel; Osteoarthritis; MICROSPHERES; METABOLISM; AUTOPHAGY; CHITOSAN; SCAFFOLD; RELEASE;
D O I
10.1016/j.cej.2025.159529
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Osteoarthritis (OA) is closely related to articular cartilage injury and joint inflammation. L-Glutamine (L-Gln) plays a significant role in delaying articular cartilage degeneration and reducing inflammation. However, the molecular weight of L-Gln is small and its half-life in vivo is relatively short, which limits its therapeutic effect. In this study, gelatin microspheres coated with L-Gln (L-Gln@GMs) were combined with a quaternized chitosanpolyvinyl alcohol self-assembled hydrogel to prepare a composite hydrogel (L-Gln@GMs@QCSFP) that responds to an inflammatory microenvironment. The hydrogel has good mechanical properties and injectability and can be adsorbed onto the cartilage surface to respond to a high reactive oxygen species environment, thus realizing the slow and stable release of L-Gln. In in vitro experiments, the L-Gln@GMs@QCSFP hydrogel exhibited good antibacterial properties and biocompatibility, and effectively ameliorated the chondrocyte damage induced by interleukin (IL)-1(3 by inhibiting the IL-17 and PI3K/Akt signaling pathways. In addition, LGln@GMs@QCSFP also promoted polarization of macrophages from M1 type to M2 type. In an OA rat model, the L-Gln@GMs@QCSFP hydrogel also effectively delayed cartilage injury and improved synovitis. This approach is expected to provide an effective and safe new strategy for the treatment of OA.
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页数:21
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