Boron carbide nanoparticles for boron neutron capture therapy

被引:0
作者
Xu, Shiwei [1 ]
Yu, Ying [1 ]
Zhang, Boyu [1 ]
Zhu, Kejia [3 ]
Cheng, Yuan [3 ]
Zhang, Tao [1 ,2 ,3 ,4 ]
机构
[1] Nanjing Univ, Coll Engn & Appl Sci, Nanjing 210023, Peoples R China
[2] Nanjing Univ, MOE Key Lab High Performance Polymer Mat & Technol, Nanjing 210023, Peoples R China
[3] Wuxi Xishan NJU Inst Appl Biotechnol, Wuxi 214104, Peoples R China
[4] Qinghai Inst Technol, Sch Engn, Xining 810016, Peoples R China
基金
国家重点研发计划;
关键词
FOLATE RECEPTOR; CARBON DOTS; FOLIC-ACID; BNCT; CHITOSAN; TUMORS;
D O I
10.1039/d5ra00734h
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Boron agent is widely accepted as one of the most important factors in boron neutron capture therapy (BNCT). In this study, boron carbide (B4C) nanoparticles were subjected to chemical modification, with the folic acid moiety linked to the surface of the particles by varying the segments of the covalent linker polyethylene glycol (PEG) through gamma-aminopropyltriethoxysilane (APTES) functionalization. The resultant products were three boron agents, termed as B4C-APTES-FA, B4C-APTES-PEG2K-FA, and B4C-APTES-PEG5K-FA. A comparison was made between these products and the pristine B4C nanoparticles by investigating their physicochemical properties and biological performances, including hemolysis, cytotoxicity, and cellular uptake. Subsequently, the modified B4C-APTES-PEG2K-FA nanoparticles were subjected to in vivo safety assays and biodistribution investigations in mice at various dosages. Upon characterization using ICP-OES, it was found that the boron contents were the highest in the lungs, followed by the liver, spleen, kidneys, hearts, and tumors, and the lowest in the brain and muscles. The boron content in the tumor reached as high as 50 mu g per g of dried tissue weight after 24 h of intravenous injection (I.V.), while the tumor-to-muscle and tumor-to-brain ratios of boron contents were found to exceed 3 following 24 hours of intravenous injection. These findings suggest that B4C nanoparticles are promising for BNCT owing to their high boron content, satisfactory biocompatibility, and abundant chemical modification sites.
引用
收藏
页码:10717 / 10730
页数:14
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