Protocol for the preparation of zebrafish whole heart cell suspension for single-cell analyses

被引：0
|
作者：
Abu Nahia, Karim [1 ]
Winata, Cecilia Lanny [1 ]
机构：
[1] Int Inst Mol & Cell Biol Warsaw, PL-02109 Warsaw, Poland
来源：
STAR PROTOCOLS | 2025年 / 6卷 / 01期
关键词：
REVEALS;
D O I：
10.1016/j.xpro.2024.103586
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Obtaining viable cell suspension that accurately represents the diversity of complex tissues is challenging due to the distinct characteristics of each cell type. Here, we present a protocol for preparing a single-cell suspension of the zebra- fish embryonic whole heart, detailing steps for heart extraction, cell dissociation, quantification, and quality assessment. This suspension is compatible with downstream analysis on various single-cell platforms. For details on the use and execution of this protocol, please refer to Abu Nahia et al.1
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页数：11
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