A function of TPL/TBL1-type corepressors is to nucleate the assembly of the preinitiation complex

被引：1

作者：

Leydon, Alexander R. ^{[1
]}

Downing, Benjamin ^{[1
]}

Sanchez, Janet Solano ^{[1
]}

Loll-Krippleber, Raphael ^{[3
,4
]}

Belliveau, Nathan M. ^{[1
]}

Rodriguez-Mias, Ricard A. ^{[2
]}

Bauer, Andrew J. ^{[1
]}

Watson, Isabella J. ^{[1
]}

Bae, Lena ^{[1
]}

Villen, Judit ^{[2
]}

Brown, Grant W. ^{[3
,4
]}

Nemhauser, Jennifer L. ^{[1
]}

机构：

[1] UNIV WASHINGTON, Dept Biol, Seattle, WA 98195 USA

[2] UNIV WASHINGTON, Dept Genome Sci, Seattle, WA USA

[3] Univ Toronto, Dept Biochem, Toronto, ON, Canada

[4] Univ Toronto, Donnelly Ctr, Toronto, ON, Canada

来源：

JOURNAL OF CELL BIOLOGY | 2024年 / 224卷 / 02期

基金：

美国国家卫生研究院;

关键词：

RNA-POLYMERASE-II; N-TERMINAL DOMAIN; TRANSCRIPTION ELONGATION; SACCHAROMYCES-CEREVISIAE; GENE-EXPRESSION; SPT4-SPT5; COMPLEX; CRYSTAL-STRUCTURE; CO-REPRESSORS; PROTEIN; YEAST;

D O I：

10.1083/jcb.202404103

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The plant corepressor TPL is recruited to diverse chromatin contexts, yet its mechanism of repression remains unclear. Previously, we leveraged the fact that TPL retains its function in a synthetic transcriptional circuit in the yeast model Saccharomyces cerevisiae to localize repressive function to two distinct domains. Here, we employed two unbiased whole-genome approaches to map the physical and genetic interactions of TPL at a repressed locus. We identified SPT4, SPT5, and SPT6 as necessary for repression with SPT4 acting as a bridge connecting TPL to SPT5 and SPT6. We discovered the association of multiple additional constituents of the transcriptional preinitiation complex at TPL-repressed promoters, specifically those involved early in transcription initiation. These findings were validated in yeast and plants, including a novel method to analyze the conditional loss of function of essential genes in plants. Our findings support a model where TPL nucleates preassembly of the transcription activation machinery to facilitate the rapid onset of transcription once repression is relieved.

引用

页数：29