Optimizing natural human-derived decellularized tissue materials for periodontal bone defect repair

被引：0

作者：

Zhang, Xiao-Hui ^{[1
,2
,3
,4
]}

Wang, Hao ^{[1
,2
,3
]}

Cao, Yuan ^{[1
,2
,3
,4
]}

Liu, Lu ^{[1
,2
,3
,4
]}

Zhang, Zi-Qi ^{[1
,2
,3
,4
]}

Liu, Jia-Ning ^{[1
,2
,3
,4
]}

Mu, Shi-Han ^{[1
,2
,3
,4
]}

Yuan, Yuan ^{[1
,2
,3
]}

Cai, Xin-Yue ^{[1
,2
,3
,5
]}

Sui, Bing-Dong ^{[1
,2
,3
]}

Zheng, Chen-Xi ^{[1
,2
,3
]}

Xu, Hao-Kun ^{[1
,2
,3
]}

Jin, Fang ^{[4
]}

Lei, Xiao ^{[1
,2
,3
,4
]}

机构：

[1] Fourth Mil Med Univ, Ctr Tissue Engn, Sch Stomatol, State Key Lab Oral & Maxillofacial Reconstruct & R, Xian 710032, Shaanxi, Peoples R China

[2] Fourth Mil Med Univ, Natl Clin Res Ctr Oral Dis, Sch Stomatol, Xian 710032, Shaanxi, Peoples R China

[3] Fourth Mil Med Univ, Shaanxi Int Joint Res Ctr Oral Dis, Ctr Tissue Engn, Sch Stomatol, Xian 710032, Shaanxi, Peoples R China

[4] Fourth Mil Med Univ, Sch Stomatol, Dept Orthodont, Xian 710032, Shaanxi, Peoples R China

[5] Fourth Mil Med Univ, Sch Stomatol, Dept Prevent Dent, Xian 710032, Shaanxi, Peoples R China

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2025年 / 748卷

基金：

中国博士后科学基金; 中国国家自然科学基金;

关键词：

Decellularized matrix; Periodontal disease; Bone defect; Tissue regeneration; Stem cells; Microparticles; EXTRACELLULAR-MATRIX; DENTIN MATRIX; GRAFTS; CELLS; BIOMATERIALS; REGENERATION;

D O I：

10.1016/j.bbrc.2025.151297

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Periodontal disease is a major contributor to tooth loss worldwide in adults. Particularly, periodontal bone defect is a common clinical condition, yet current therapeutic strategies exhibit limited effectiveness. Recently, natural bone graft materials have attracted considerable interest for enhancing bone defect repair due to their superior biocompatibility and osteogenic capabilities. Nevertheless, clinically applicable human-derived biomaterials to boost bone regeneration are currently not accessible. Here, enlightened by the decellularization technique, we successfully prepared the human decellularized alveolar bone and tooth dentin tissues from healthy individuals and obtained micro-sized bioactive decellularized extracellular matrix (dECM) particles for treatment of periodontal bone defects. After characterizing the two representative dECM tissues and particles by multiple physiochemical approaches, we revealed that both human decellularized alveolar bone matrix particles (hDABMPs) and human decellularized tooth dentin matrix particles (hDTDMPs) possessed excellent biocompatibility. Furthermore, both biomaterials significantly enhanced the proliferation and osteogenic differentiation of human dental follicle stem cells, potentially contributing to periodontal bone formation. Indeed, in a rat model, both types of dECM microparticles were found to facilitate tissue regeneration at periodontal bone defect sites, demonstrating comparable efficacy for promoting bone defect repair. Collectively, this study provides an important basis for clinical exploration of natural human-derived micro-sized biomaterials for periodontal bone defect repair and opens a new path for periodontal disease treatment strategies.

引用

页数：8

共 38 条

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