Cyclin-dependent kinases (CDKs) are key genes regulating early development of Neptunea arthritica cumingii: evidence from comparative transcriptome and proteome analyses

被引:0
作者
Lv, Fengxiao [1 ]
Ge, Xinfan [1 ]
Chang, Yaqing [1 ,2 ]
Hao, Zhenlin [1 ,2 ]
机构
[1] Dalian Ocean Univ, Minist Agr, Key Lab Mariculture & Stock Enhancement North Chin, Dalian 116023, Peoples R China
[2] Dalian Ocean Univ, Coll Fisheries & Life Sci, 52 Heishijiao St, Dalian, Liaoning, Peoples R China
来源
BMC GENOMICS | 2024年 / 25卷 / 01期
基金
中国国家自然科学基金;
关键词
N; cumingii; CDKs; Transcriptome; Proteome; Early development; PROTEOGENOMIC CHARACTERIZATION; GENOME ANNOTATION; RAPANA-VENOSA; EXPRESSION; P27;
D O I
10.1186/s12864-024-10970-3
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
In this study, we applied comparative transcriptomics and proteomics techniques to systematically investigate the dynamic expression patterns of genes and proteins at various stages of early embryonic development of the gastropod Neptunea arthritica cumingii. Twelve cyclin-dependent kinase (CDKs) genes and five downstream proteins associated with these CDKs were identified. Through techniques such as qRT-PCR, our data elucidate for the first time the regulatory functions of CDK family genes and establish CDKs as a pivotal gene cluster in the early embryonic development of N. cumingii. These findings not only enhance the understanding of molecular developmental biology in N. cumingii and marine gastropods in general but also provide significant insights into the mechanisms involved in early embryonic development in N. cumingii. Furthermore, our results provide theoretical guidance for advancing artificial breeding technology for N. cumingii.
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页数:17
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