Brief guide to RT-qPCR

被引：13

作者：

Bong, Dajeong ^{[1
]}

Sohn, Jooyeon ^{[1
]}

Lee, Seung-Jae, V ^{[1
]}

机构：

[1] Korea Adv Inst Sci & Technol, Dept Biol Sci, 291 Daehak Ro, Daejeon 34141, South Korea

来源：

MOLECULES AND CELLS | 2024年 / 47卷 / 12期

基金：

新加坡国家研究基金会;

关键词：

Reverse transcription-quantitative polymerase chain reaction; RNA quantification; REVERSE-TRANSCRIPTION; PCR; INHIBITION;

D O I：

10.1016/j.mocell.2024.100141

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

RNA quantification is crucial for understanding gene expression and regulation. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) is a widely used technique for RNA quantification because of its practical and quantitative nature, sensitivity, and specificity. Here, we provide an overview of RT-qPCR, focusing on essential reagents, the importance of primer design, the detailed workflow, and data analysis methods. This guide will be useful for scientists who are unfamiliar with RT-qPCR, highlighting key considerations for successful RNA quantification. (c) 2024 The Author(s). Published by Elsevier Inc. on behalf of Korean Society for Molecular and Cellular Biology. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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页数：5

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