LncRNA THUMPD3-AS1/microRNA-4465/KPNA2 axis impacts human hepatocellular carcinoma cell phenotypes

被引：0
作者：
Wang, Jiawei [1 ]
Qiao, Chunzhong [1 ]
Luo, Baoyang [1 ]
Qin, Lei [2 ]
机构：
[1] Nanjing Med Univ, Taizhou Peoples Hosp, Dept Hepatobiliary & Pancreat Surg, Taizhou 225300, Mainland, Peoples R China
[2] Soochow Univ, Affiliated Hosp 1, Dept Hepatobiliary & Pancreat Surg, 899 Pingxi Rd, Suzhou 215000, Jiangsu, Peoples R China
来源：
REGENERATIVE THERAPY | 2025年 / 28卷
关键词：
Hepatocellular carcinoma; LncRNA THUMPD3-AS1; MicroRNA-4465; KPNA2; Apoptosis; Migration; LUNG-CANCER; PROLIFERATION; PROGRESSION; MIGRATION; INVASION;
D O I：
10.1016/j.reth.2025.01.010
中图分类号：
Q813 [细胞工程];
学科分类号：
摘要：
Objective: Hepatocellular carcinoma (HCC) is a lethal malignancy in the world. LncRNA THUMPD3-AS1 is implicated in tumorigenesis and progression in various tumors. Therefore, this study was applied to investigate the action of THUMPD3-AS1 in HCC by regulating microRNA (miR)-4465 and KPNA2. Methods: The clinical specimens of HCC were collected to determine THUMPD3-AS1, KPNA2, miR-4465, E-cadherin, Vimentin, N-cadherin, ZEB1 and SNAIL levels. HCC cells were screened and transfected with sh-THUMPD3-AS1 or miR-4465 mimic to explore their roles in HCC cell phenotype and epithelial-mesenchymal transition (EMT)-related factors. The involvement of miR-4465 in THUMPD3-AS1-mediated HCC was proved. The relationship of THUMPD3-AS1, KPNA2 and miR-4465 was verified. Results: Overexpressed THUMPD3-AS1 and KPNA2 and reduced miR-4465 were present in HCC clinical tissues. THUMPD3-AS1 bound to miR-4465 to target KPNA2. Silencing of THUMPD3-AS1 or restoration of miR-4465 repressed HCC cell phenotypes and EMT in vitro. Inhibition of miR-4465 mitigated the role of silenced THUMPD3-AS1 in HCC. Conclusion: This study stresses that THUMPD3-AS1 induces EMT in HCC cells and ultimately promotes HCC cell growth and migration by competitively inhibiting miR-4465 expression and thus upregulating KPNA2. (c) 2025 The Author(s). Published by Elsevier BV on behalf of The Japanese Society for Regenerative Medicine.
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收藏
页码：413 / 420
页数：8
相关论文
共 31 条
[1]   LINC00240 sponges miR-4465 to promote proliferation, migration, and invasion of hepatocellular carcinoma cells via HGF/c-MET signaling pathway [J].
Bu, W-J ;
Fang, Z. ;
Li, W-L ;
Wang, X. ;
Dong, M-J ;
Tao, Q-Y ;
Zhang, L. ;
Xu, Y-Q .
EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES, 2020, 24 (20) :10452-10461
[2]   The effect of medium supplementation and serial passaging on the transcriptome of human adipose-derived stromal cells expanded in vitro [J].
Dessels, Carla ;
Ambele, Melvin A. ;
Pepper, Michael S. .
STEM CELL RESEARCH & THERAPY, 2019, 10 (01)
[3]   Intranasal Mesenchymal Stem Cell Treatment for Neonatal Brain Damage: Long-Term Cognitive and Sensorimotor Improvement [J].
Donega, Vanessa ;
van Velthoven, Cindy T. J. ;
Nijboer, Cora H. ;
van Bel, Frank ;
Kas, Martien J. H. ;
Kavelaars, Annemieke ;
Heijnen, Cobi J. .
PLOS ONE, 2013, 8 (01)
[4]   Regulation of microRNA function in animals [J].
Gebert, Luca F. R. ;
MacRae, Ian J. .
NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2019, 20 (01) :21-37
[5]   The emerging roles of KPNA2 in cancer [J].
Han, Yang ;
Wang, Xin .
LIFE SCIENCES, 2020, 241
[6]   THUMPD3-ASI Is Correlated With Non-Small Cell Lung Cancer And Regulates Self-Renewal Through miR-543 And ONECUT2 [J].
Hu, Jia ;
Chen, Youfang ;
Li, Xiaodong ;
Miao, Huikai ;
Li, Rongzhen ;
Chen, Dongni ;
Wen, Zhesheng .
ONCOTARGETS AND THERAPY, 2019, 12 :9849-9860
[7]   MicroRNA-98-5p Inhibits Cell Proliferation and Induces Cell Apoptosis in Hepatocellular Carcinoma via Targeting IGF2BP1 [J].
Jiang, Tinghui ;
Li, Mengfan ;
Li, Qiuyin ;
Guo, Zhiqiang ;
Sun, Xianjun ;
Zhang, Xufeng ;
Liu, Yan ;
Yao, Wenyi ;
Xiao, Ping .
ONCOLOGY RESEARCH, 2017, 25 (07) :1117-1127
[8]   RETRACTED: LncRNA XIST functions as a molecular sponge of miR-194-5p to regulate MAPK1 expression in hepatocellular carcinoma cell (Retracted article. See vol. 122, pg. 1761, 2021) [J].
Kong, Qinglei ;
Zhang, Shaoquan ;
Liang, Caiqian ;
Zhang, Ying ;
Kong, Qingcong ;
Chen, Shuxian ;
Qin, Jie ;
Jin, Yi .
JOURNAL OF CELLULAR BIOCHEMISTRY, 2018, 119 (06) :4458-4468
[9]   Identification and Functional Characterization of Two Noncoding RNAs Transcribed from Putative Active Enhancers in Hepatocellular Carcinoma [J].
Lee, Ye-Eun ;
Lee, Jiyeon ;
Lee, Yong Sun ;
Jang, Jiyoung Joan ;
Woo, Hyeonju ;
Choi, Hae In ;
Chai, Young Gyu ;
Kim, Tae-Kyung ;
Kim, TaeSoo ;
Kim, Lark Kyun ;
Choi, Sun Shim .
MOLECULES AND CELLS, 2021, 44 (09) :658-669
[10]   CACYBP Enhances Cytoplasmic Retention of P27Kip1 to Promote Hepatocellular Carcinoma Progression in the Absence of RNF41 Mediated Degradation [J].
Lian, Yi-Fan ;
Huang, Yan-Lin ;
Zhang, Yao-Jun ;
Chen, Dong-Mei ;
Wang, Jia-Liang ;
Wei, Huan ;
Bi, Yan-Hua ;
Jiang, Zhi-Wu ;
Li, Peng ;
Chen, Min-Shan ;
Huang, Yue-Hua .
THERANOSTICS, 2019, 9 (26) :8392-8408
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