Structural basis for human DPP4 receptor recognition by a pangolin MERS-like coronavirus

被引：0

作者：

Yang, Mo ^{[1
]}

Li, Zehou ^{[1
]}

Chen, Jing ^{[2
]}

Li, Yang ^{[2
]}

Xu, Ran ^{[3
]}

Wang, Meihua ^{[2
]}

Xu, Ying ^{[1
]}

Chen, Rong ^{[4
]}

Ji, Weiwei ^{[1
]}

Li, Xiaoxia ^{[1
]}

Wei, Jiayu ^{[1
]}

Zhou, Zhengrong ^{[1
]}

Ren, Minjie ^{[1
]}

Ma, Ke ^{[1
]}

Guan, Jiayu ^{[1
]}

Mo, Guoxiang ^{[1
]}

Zhou, Peng ^{[5
]}

Shu, Bo ^{[2
]}

Guo, Jingjing ^{[3
]}

Yuan, Yuan ^{[6
]}

Shi, Zheng-Li ^{[2
]}

Zhang, Shuijun ^{[1
]}

机构：

[1] Nanjing Agr Univ, Coll Life Sci, Nanjing, Peoples R China

[2] Chinese Acad Sci, Wuhan Inst Virol, Key Lab Virol & Biosafety, Wuhan, Peoples R China

[3] Macao Polytech Univ, Fac Appl Sci, Ctr Artificial Intelligence Driven Drug Discovery, Taipa, Macao, Peoples R China

[4] Jiangsu Acad Agr Sci, Inst Vet Med, Key Lab Vet Biol Engn & Technol, Minist Agr, Nanjing, Peoples R China

[5] Guangzhou Int Bio Isl, Guangzhou Lab, Guangzhou, Guangdong, Peoples R China

[6] Anhui Univ, Sch Life Sci, Hefei, Anhui, Peoples R China

来源：

PLOS PATHOGENS | 2024年 / 20卷 / 11期

基金：

中国博士后科学基金; 中国国家自然科学基金;

关键词：

RESPIRATORY SYNDROME CORONAVIRUS; BAT; BINDING; COV; BETACORONAVIRUS; DOMAIN;

D O I：

10.1371/journal.ppat.1012695

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Middle East respiratory syndrome coronavirus (MERS-CoV) and the pangolin MERS-like coronavirus MjHKU4r-CoV-1 employ dipeptidyl peptidase 4 (DPP4) as an entry receptor. MjHKU4r-CoV-1 could infect transgenic mice expressing human DPP4. To understand the mechanism of MjHKU4r-CoV-1 entry into cells, we determined the crystal structures of the receptor binding domain (RBD) of MjHKU4r-CoV-1 spike protein bound to human DPP4 (hDPP4) and Malayan pangolin DPP4 (MjDPP4), respectively. The overall hDPP4-binding mode of MjHKU4r-CoV-1 RBD is similar to that of MERS-CoV RBD. MjHKU4r-CoV-1 RBD shows higher binding affinity to hDPP4 compared to the bat MERS-like coronavirus Ty-BatCoV-HKU4. Via swapping residues between MjHKU4r-CoV-1 RBD and Ty-BatCoV-HKU4 RBD, we identified critical determinants on MjHKU4r-CoV-1 that are responsible for virus usage of hDPP4. Our study suggests that MjHKU4r-CoV-1 is more adapted to the human receptor compared to the bat HKU4 coronavirus and highlights the potential of virus emergence into the human population.

引用

页数：19

共 27 条

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