TGF-β effects on adipogenesis of 3T3-L1 cells differ in 2D and 3D cell culture conditions

被引：0

作者：

Umetsu, Araya ^{[1
]}

Watanabe, Megumi ^{[1
]}

Sato, Tatsuya ^{[2
,3
]}

Higashide, Megumi ^{[1
]}

Nishikiori, Nami ^{[1
]}

Furuhashi, Masato ^{[2
]}

Ohguro, Hiroshi ^{[1
]}

机构：

[1] Sapporo Med Univ, Sch Med, Dept Ophthalmol, Minami 1 Jo Nishi 16 Chome,Chuo Ku, Sapporo, Hokkaido 0608543, Japan

[2] Sapporo Med Univ, Sch Med, Dept Cardiovasc Renal & Metab Med, Sapporo, Japan

[3] Sapporo Med Univ, Sch Med, Dept Cellular Physiol & Signal Transduct, Sapporo, Japan

来源：

FEBS OPEN BIO | 2024年 / 14卷 / 12期

关键词：

3D culture; adipogenesis; TGF-beta isoform; TRANSFORMING-GROWTH-FACTOR; ACTIVATED RECEPTOR-GAMMA; GENE-EXPRESSION; ADIPOSE-TISSUE; STEM-CELLS; DIFFERENTIATION; TGF-BETA(1); FIBROBLASTS; ADIPOCYTES; INDUCTION;

D O I：

10.1002/2211-5463.13890

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The TGF-beta superfamily plays a pivotal role in the regulation of adipogenesis, but little is known about the potential differential role of the three isoforms of TGF-beta, TGF-beta-1 similar to 3. To further elucidate their role, two-dimensionally (2D) and three-dimensionally (3D) cultured 3T3-L1 mouse preadipocytes were subjected to the following analyses: (a) qPCR analysis of adipogenesis-related factors and major extracellular matrix protein (2D and /or 3D), (b) lipid staining by Oil Red O (2D) or BODIPY (3D), (c) Seahorse cellular metabolic measurement (2D), and (d) size and stiffness measurements of 3D 3T3-L1 spheroids. In the 2D cultured 3T3-L1 cells, mRNA expression levels of adipogenesis-related genes and Oil Red O lipid staining intensity were significantly increased by adipogenesis and they were substantially decreased following treatment with 0.1 nm TGF-beta isoforms, with TGF-beta 2 having the greater effects. Consistent with these results, treatment with TGF-beta 2 resulted in suppression of mitochondrial and glycolytic functions in 2D cultured 3T3-L1 cells. However, the inhibitory effect of TGF-beta on adipogenesis decreased under 3D spheroid culture conditions and TGF-beta isoforms did not affect adipogenesis-induced (a) enlargement and downsizing of 3T3-L1 spheroids, (b) increase in BODIPY lipid staining intensity, and (c) up-regulation of the mRNA expression of adipogenesis-related genes. The findings presented herein suggest that the three TGF-beta isoforms have different suppressive effects on adipogenesis-related cellular properties of 2D cultured 3T3-L1 cells and that their effects decrease under 3D spheroid culture conditions.

引用

页码：2026 / 2037

页数：12