Phenotypic Characterization and Isolation of Myeloid-Derived Suppressor Cells

被引：1

作者：

Reuven, Or ^{[1
]}

Mikula, Ivan, Jr. ^{[1
]}

Ashkenazi-Preiser, Hadas ^{[1
]}

Twaik, Nira ^{[1
]}

Ben-Meir, Kerem ^{[1
]}

Meirow, Yaron ^{[1
]}

Daniel, Leonor ^{[1
]}

Kariv, Guy ^{[1
]}

Kurd, Mahdi ^{[1
]}

Baniyash, Michal ^{[1
]}

机构：

[1] Hebrew Univ Jerusalem, Lautenberg Ctr Immunol & Canc Res, Israel Canada Med Res Inst, Concern Fdn Labs,Fac Med, Jerusalem, Israel

来源：

CURRENT PROTOCOLS | 2022年 / 2卷 / 10期

基金：

以色列科学基金会;

关键词：

chronic inflammation; flow cytometry; immunofluorescence imaging; immunosuppression; inflammatory mouse model; myeloid-derived suppressor cells; MDSC; patient MDSC;

D O I：

10.1002/cpz1.561

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Myeloid-derived suppressor cells (MDSCs) are heterogenous populations of immature myeloid cells that can be divided into two main subpopulations, polymorphonuclear (PMN) MDSCs and monocytic (M) MDSCs. These cells accumulate during chronic inflammation and induce immunosuppression evident in an array of pathologies such as cancer, inflammatory bowel disease, and infectious and autoimmune diseases. Herein, we describe methods to isolate and characterize MDSCs from various murine tissue, as well as to phenotype blood-derived MDSCs from patients. The protocols describe methods for isolation of total MDSCs and their subpopulations, for characterization, and for evaluation of their distribution within tissue, as well as for assessing their maturation stage by flow cytometry, immunofluorescence analyses, and Giemsa staining. (c) 2022 The Authors. Current Protocols published by Wiley Periodicals LLC.

引用

页数：31

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