Long-term and low-dose exposure to triclosan induces POI phenotype in female offspring mice

被引：0

作者：

Gan, Hongya ^{[1
]}

Jiang, Yan ^{[1
]}

Wu, Lixiang ^{[1
]}

Zhu, Bingqi ^{[1
]}

Ji, Dapeng ^{[2
]}

Liu, Jing ^{[3
]}

Ding, Zhishan ^{[1
]}

Ye, Xiaoqing ^{[1
]}

机构：

[1] Zhejiang Chinese Med Univ, Sch Med Technol & Informat Engn, Hangzhou 310053, Zhejiang, Peoples R China

[2] Zhejiang Chinese Med Univ, Logist Management Off, Hangzhou 310053, Zhejiang, Peoples R China

[3] Zhejiang Univ, Coll Environm & Resource Sci, MOE Key Lab Environm Remediat & Ecosyst Hlth, Hangzhou 310058, Peoples R China

来源：

ENVIRONMENTAL POLLUTION | 2025年 / 372卷

基金：

中国国家自然科学基金;

关键词：

Triclosan; POI phenotype; Apoptosis; Gut microbiota; Metabolism; BISPHENOL-A; INFANT; TRICLOCARBAN; PREGNANCY; PHENOLS; CYCLE;

D O I：

10.1016/j.envpol.2025.125966

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Triclosan (TCS), a typical endocrine disruptor, is widely used as an antibacterial agent in consumer goods. However, there are few studies on the effects of long-term low-dose TCS exposure on ovarian function in F1 female mice. In this paper, F1 female mice were exposed to TCS (0-3000 mu g/kg/day) from intrauterine to postnatal day (PND) 91 to investigate its effects on the ovary. The results revealed that the number of total follicles was decreased, while atretic follicles was increased after TCS exposure. At the hormonal level, the secretion of estradiol was reduced, while follicle-stimulating hormone and luteinizing hormone were increased after TCS exposure. Observation of vaginal smear showed that TCS disrupted the estrous cycle of F1 female mice, especially at the dose of 3000 mu g/kg/day. Moreover, TCS promoted cell apoptosis by activating the p38-MAPK signaling pathway and oxidative stress in vitro. In addition, analysis of the fecal microbiome and serum metabolomics revealed that exposure to TCS may cause gut microbiota disruption and metabolic abnormalities in F1 female mice. In conclusion, long-term low-dose TCS exposure may induce primary ovarian insufficiency phenotype in F1 female mice via inducing cell apoptosis and disrupting gut microbiota and metabolism.

引用

页数：13

共 65 条

[31] Maeda K., Adachi S., Inoue K., Ohkura S., Tsukamura H., Metastin/kisspeptin and control of estrous cycle in rats, Rev. Endocr. Metab. Disord., 8, 1, pp. 21-29, (2007)
[32] Meli R., Monnolo A., Annunziata C., Pirozzi C., Ferrante M.C., Oxidative stress and BPA toxicity: an antioxidant approach for male and female reproductive dysfunction, Antioxidants, 9, 5, (2020)
[33] Minguez-Alarcon L., Christou G., Messerlian C., Williams P.L., Carignan C.C., Et al., Urinary triclosan concentrations and diminished ovarian reserve among women undergoing treatment in a fertility clinic, Fertil. Steril., 108, 2, pp. 312-319, (2017)
[34] Narrowe A.B., Albuthi-Lantz M., Smith E.P., Bower K.J., Roane T.M., Et al., Perturbation and restoration of the fathead minnow gut microbiome after low-level triclosan exposure, Microbiome, 3, (2015)
[35] Park H.J., Song B.S., Kim J.W., Yang S.G., Kim S.U., Et al., Exposure of triclosan in porcine oocyte leads to superoxide production and mitochondrial-mediated apoptosis during in vitro maturation, Int. J. Mol. Sci., 21, 9, (2020)
[36] Patel S., Zhou C., Rattan S., Flaws J.A., Effects of endocrine-disrupting chemicals on the ovary, Biol. Reprod., 93, 1, (2015)
[37] Revelli A., Delle Piane L., Casano S., Molinari E., Massobrio M., Et al., Follicular fluid content and oocyte quality: from single biochemical markers to metabolomics, Reprod. Biol. Endocrinol., 7, (2009)
[38] Ribado J.V., Ley C., Haggerty T.D., Tkachenko E., Bhatt A.S., Et al., Household triclosan and triclocarban effects on the infant and maternal microbiome, EMBO Mol. Med., 9, 12, pp. 1732-1741, (2017)
[39] Rolaki A., Drakakis P., Millingos S., Loutradis D., Makrigiannakis A., Novel trends in follicular development, atresia and corpus luteum regression: a role for apoptosis, Reprod. Biomed. Online, 11, 1, pp. 93-103, (2005)
[40] Ruszkiewicz J.A., Li S., Rodriguez M.B., Aschner M., Is Triclosan a neurotoxic agent?, J. Toxicol. Environ. Health B Crit. Rev., 20, 2, pp. 104-117, (2017)

← 1 2 3 4 5 6 7 →