A multi-center study on genetic variations in the fusion protein of respiratory syncytial virus from children with Acute Lower Respiratory Tract Infections in China during 2017-2021

被引:0
|
作者
Fu, Yiliang [1 ,2 ]
Li, Fei [1 ,2 ]
Zhu, Yun [1 ,2 ]
Huang, Luci [1 ,2 ]
Li, Qiuping [1 ,2 ]
Zhang, Hanwen [1 ,2 ]
Zhong, Lili [3 ]
Zhang, Hailin [4 ,5 ]
Luo, Zheng-xiu [6 ]
Lu, Gen [7 ]
Deng, Jikui [8 ]
Cao, Lingfeng [9 ]
Wu, Ying [10 ]
Jin, Rong [11 ]
Li, Lei [12 ]
Xu, Lili [1 ,2 ]
Chen, Xiangpeng [1 ,2 ]
Xie, Zhengde [1 ,2 ]
机构
[1] Capital Med Univ, Key Lab Major Dis Children, Beijing Key Lab Pediat Resp Infect Dis, Natl Clin Res Ctr Resp Dis,Natl Key Discipline Ped, Beijing 100045, Peoples R China
[2] Chinese Acad Med Sci, Res Unit Crit Infect Children, Beijing 100045, Peoples R China
[3] Hunan Normal Univ, Hunan Prov Peoples Hosp, Affiliated Hosp 1, Changsha 410005, Peoples R China
[4] Wenzhou Med Univ, Affiliated Hosp 2, Dept Childrens Respirat Dis, Wenzhou 325027, Peoples R China
[5] Wenzhou Med Univ, Yuying Childrens Hosp, Wenzhou 325027, Peoples R China
[6] Chongqing Med Univ, Natl Clin Res Ctr Child Hlth & Disorders, Dept Resp Med, Minist Educ,Key Lab Child Dev & Disorders,Chongqin, Chongqing 400015, Peoples R China
[7] Guangzhou Women & Childrens Med Ctr, Guangzhou 510623, Peoples R China
[8] Shenzhen Childrens Hosp, Dept Infect Dis, Shenzhen 518026, Peoples R China
[9] Fudan Univ, Dept Clin Lab, Childrens Hosp, Shanghai 201102, Peoples R China
[10] Shanghai Jiao Tong Univ, Natl Childrens Med Ctr, Shanghai Childrens Med Ctr, Sch Med,Dept Clin Lab Med, Shanghai 200127, Peoples R China
[11] Guiyang Maternal & Child Hlth Care Hosp, Guiyang 550003, Peoples R China
[12] Yinchuan Maternal & Child Hlth Care Hosp, Yinchuan 750001, Peoples R China
关键词
Human respiratory syncytial virus (RSV); Children; Fusion glycoprotein; Antigenic epitope; Variation;
D O I
10.1016/j.virs.2024.09.002
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Respiratory syncytial virus (RSV) is a significant cause of acute lower respiratory tract infection (ALRTI) in children under five years of age. Between 2017 and 2021, 396 complete sequences of the RSV F gene were obtained from 500 RSV-positive throat swabs collected from ten hospitals across nine provinces in China. In addition, 151 sequences from China were sourced from GenBank and GISAID, making a total of 549 RSV F gene sequences subjected to analysis. Phylogenetic and genetic diversity analyses revealed that the RSV F genes circulating in China from 2017 to 2021 have remained relatively conserved, although some amino acids (AAs) have undergone changes. AA mutations with frequencies > 10% were identified at six sites and the p27 region: V384I (site I), N276S (site II), R213S (site & Oslash;), and K124N (p27) for RSV A; F45L (site I), M152I/L172Q/S173 L/ I185V/K191R (site V), and R202Q/I206M/Q209R (site & Oslash;) for RSV B. Comparing mutational frequencies in RSV-F before and after 2020 revealed minor changes for RSV A, while the K191R, I206M, and Q209R frequencies increased by over 10% in RSV B. Notably, the nirsevimab-resistant mutation, S211N in RSV B, increased in frequency from 0% to 1.15%. Both representative strains aligned with the predicted RSV-F structures of their respective prototypes exhibited similar conformations, with low root-mean-square deviation values. These results could provide foundational data from China for the development of RSV mAbs and vaccines.
引用
收藏
页码:727 / 736
页数:10
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