Live Aspergillus cristatum from Fuzhuan Brick Tea Alleviates DSS-Induced Colitis by Intestinal Barrier Restoration and Suppressing NLRP3 Signaling Pathway Regulation

被引：0
作者：
Wang, Xin [1 ,2 ,3 ]
Cheng, Miaomiao [1 ]
Liu, Jinhu [1 ,2 ]
Guo, Yaodong [1 ,2 ,3 ]
Zhang, Yuxiang [4 ]
Yuan, Yahong [4 ]
Yue, Tianli [4 ]
机构：
[1] Shangluo Univ, Coll Hlth Management, Shangluo 726000, Peoples R China
[2] Shaanxi Union Res Ctr Univ & Enterprise Hlth & Wel, Shangluo 726000, Peoples R China
[3] Shangluo Hlth & Wellness Ind Res Inst, Shangluo 726000, Peoples R China
[4] Northwest Univ, Coll Food Sci & Technol, Xian 710069, Peoples R China
来源：
FOODS | 2025年 / 14卷 / 04期
关键词：
<italic>Aspergillus cristatum</italic>; intestinal barrier; tight junction; colitis; NLRP3; inflammasome; GUT; INFLAMMATION; JUNCTIONS;
D O I：
10.3390/foods14040549
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
Probiotics are considered an effective strategy for relieving DSS-induced colitis. This study investigated the protective effects and mechanisms of Aspergillus cristatum, a potential probiotic fungus from Fuzhuan brick tea, on colitis. Supplementation with live 102 spores/mL of A. cristatum H-1 and 105 spores/mL of A. cristatum S-6 significantly improved gut integrity by preventing colon shortening, mucus disruption, and goblet cell depletion. Additionally, it significantly reduced proinflammatory cytokines IL-6 and TNF-alpha levels, enhanced the expression of tight junction molecules (ZO-1, Claudin-1, E-cadherin, and MUC1) and suppressed the NLRP3 signaling pathway. Live A. cristatum H-1 (102 spores/mL) and A. cristatum S-6 (105 spores/mL) can effectively improve colitis. But the inactivated A. cristatum H-1 did not exhibit effective anti-inflammatory effects and significant interspecies differences. In a word, live low-dose A. cristatum H-1 and high-dose A. cristatum S-6 promise a valuable approach to improving colitis. This research not only enhances our understanding of probiotics and their potential therapeutic uses but also sets the stage for future investigations into the mechanisms of action and clinical utilization of A. cristatum in treating colitis and other gut disorders.
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页数：15
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