Cryosectioning

被引：3

作者：

Ross, Mark A. ^{[1
]}

Kohut, Lauryn ^{[2
]}

Loughran, Patricia A. ^{[1
,2
]}

机构：

[1] Univ Pittsburgh, Ctr Biol Imaging, Pittsburgh, PA 15260 USA

[2] Univ Pittsburgh, Dept Surg, Pittsburgh, PA 15260 USA

来源：

CURRENT PROTOCOLS | 2022年 / 2卷 / 01期

关键词：

cryopreservation; cryosectioning and immunofluorescence; tissue perfusion-fixation;

D O I：

10.1002/cpz1.342

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The protocols presented here describe steps for cryosectioning tissue samples to be used in light microscopy methodologies including histochemistry, enzyme immunohistochemistry, and immunofluorescence. (c) 2022 Wiley Periodicals LLC.

引用

页数：8