Optimizing Solid-Phase Protein Synthesis Using CPG-2000 and a Nickel-Cleavable SNAC-tag Linker

被引：0
|
作者：
Han, Jianyi [1 ,2 ,3 ,4 ,5 ]
Dang, Bobo [1 ,2 ,3 ,4 ,5 ]
机构：
[1] Zhejiang Univ, Coll Life Sci, Hangzhou 310058, Zhejiang, Peoples R China
[2] Westlake Univ, Res Ctr Ind Future, Sch Life Sci, Hangzhou 310024, Zhejiang, Peoples R China
[3] Westlake Univ, Sch Life Sci, Key Lab Struct Biol Zhejiang Prov, Hangzhou 310024, Zhejiang, Peoples R China
[4] Westlake Lab Life Sci & Biomed, Hangzhou 310024, Zhejiang, Peoples R China
[5] Westlake Inst Adv Study, Inst Biol, Hangzhou 310024, Zhejiang, Peoples R China
来源：
ORGANIC LETTERS | 2025年 / 27卷 / 09期
基金：
中国国家自然科学基金;
关键词：
TOTAL CHEMICAL-SYNTHESIS; LIGATION; PEPTIDES;
D O I：
10.1021/acs.orglett.5c00109
中图分类号：
O62 [有机化学];
学科分类号：
070303 ; 081704 ;
摘要：
Solid-phase chemical ligation (SPCL) is a powerful method for simplifying protein synthesis but faces challenges with orthogonal protection strategies, suboptimal solid supports, and limited cleavable linkers. In this study, we optimized SPCL by combining low-loading controlled-pore glass (CPG-2000) with a nickel-cleavable SNAC-tag linker. This system enabled the successful assembly of five peptide fragments and the efficient synthesis of a 131-amino-acid de novo protein, achieving a 25% yield.
引用
收藏
页码：2104 / 2109
页数：6
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