Expression and prognostic significance of the m6A RNA methylation regulator HNRNPC in HNSCC

被引：0

作者：

Zhang, Yulin ^{[1
]}

Wang, Yixu ^{[2
]}

Peng, Jilin ^{[1
]}

Zhao, Kun ^{[1
]}

Li, Ling ^{[1
]}

Zhang, Yuan ^{[1
]}

Zhai, Ziyu ^{[1
]}

Yuan, Sijie ^{[1
]}

Li, Shichao ^{[3
,4
,5
]}

Ye, Fanglei ^{[1
]}

Wang, Le ^{[1
]}

机构：

[1] Zhengzhou Univ, Dept Otolaryngol Head & Neck Surg, Affiliated Hosp 1, Zhengzhou, Peoples R China

[2] Peking Univ, Peoples Hosp, Dept Otolaryngol Head & Neck Surg, Beijing, Peoples R China

[3] Henan Prov Peoples Hosp, Dept Otolaryngol Head & Neck Surg, Zhengzhou, Henan, Peoples R China

[4] Peoples Hosp Zhengzhou Univ, Dept Otolaryngol Head & Neck Surg, Peoples Hosp, Zhengzhou, Henan, Peoples R China

[5] Henan Univ, Peoples Hosp, Dept Otolaryngol Head & Neck Surg, Zhengzhou, Henan, Peoples R China

来源：

FRONTIERS IN ONCOLOGY | 2025年 / 15卷

基金：

中国国家自然科学基金;

关键词：

heterogeneous nuclear ribonucleoprotein C; head and neck squamous cell carcinoma; prognostic significance; tumor progression; N6-methyladenosine; MESSENGER-RNA; NUCLEAR-RNA; HEAD; REVEALS; PROTEIN; YTHDC2;

D O I：

10.3389/fonc.2025.1516867

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Background N6-methyladenosine (m6A) RNA modification is crucial for tumor development and progression; however, which m6A regulators play a pivotal role in head and neck squamous cell carcinoma (HNSCC) remains ambiguous.Methods Utilizing the Cancer Genome Atlas (TCGA) database, the expression levels of m6A regulators in HNSCC were examined, which led to the identification of heterogeneous nuclear ribonucleoprotein C (HNRNPC) as a key gene. Further experiments were performed in patient samples, stable cell lines, and a murine xenograft tumor model.Results A reliable survival risk model of m6A was constructed based on the TCGA database. Gene Expression Omnibus (GEO), normal and tumor tissue microarrays (TMA), and tumor tissue samples from patients with HNSCC were observed that a high level of HNRNPC expression was closely linked to a poor prognosis among patients. Knockdown of HNRNPC in the HNSCC cell lines HSC-3 and CAL-27 resulted in a significant decrease in proliferation, invasion, and malignant transformation abilities. RNA sequencing (RNA-seq) and methylated RNA immunoprecipitation and sequencing (MeRIP-seq) data revealed that HNRNPC is involved in cell differentiation, cell migration and apoptosis. The mouse xenograft model elucidated that HNRNPC can promote tumorigenesis and progression of HNSCC.Conclusions HNRNPC can serve as a valuable predictor of tumor progression and prognosis in patients with HNSCC.

引用

页数：12

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