Label-free evaluation of mouse embryo quality using time-lapse bright field and optical coherence microscopy

被引：0

作者：

Wang, Fei ^{[1
]}

Hao, Senyue ^{[2
]}

Park, Kibeom ^{[1
]}

Ahmady, Ali ^{[3
]}

Zhou, Chao ^{[1
,2
]}

机构：

[1] Washington Univ, Dept Biomed Engn, St Louis, MO 63130 USA

[2] Washington Univ, Dept Elect & Syst Engn, St Louis, MO 63130 USA

[3] Washington Univ, Dept Obstet & Gynecol, St Louis, MO USA

来源：

COMMUNICATIONS BIOLOGY | 2025年 / 8卷 / 01期

关键词：

IMPLANTATION; TOMOGRAPHY; COMPACTION; GUIDELINES; SELECTION; DYNAMICS; ERA;

D O I：

10.1038/s42003-025-08044-5

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The selection of high-quality embryos is essential to enhance the implantation rate for in vitro fertilization (IVF). Optical coherence microscopy (OCM) can noninvasively provide three-dimensional (3D) high-resolution imaging of developing embryos. The revealed microstructures can be used for accurate embryo evaluation. Here, we acquire time-lapse 3D OCM images with co-registered bright-field imaging on mouse embryo development from the one-cell stage to the fully hatched blastocyst inside an incubator. Our results demonstrate the capability of OCM to detect structural features of the developing embryos. The second and third embryonic cell cycles are indicated to be associated with blastocyst formation and the hatching capability. OCM-based time-lapse technology holds the potential to enrich early embryo development insights and streamline embryo selection within IVF clinics.

引用

页数：12

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