Combining 2′-fucosyllactose and galacto-oligosaccharides exerts anti-inflammatory effects and promotes gut health

被引:1
作者
Park, Sewon [1 ]
Park, Yoonhee [1 ]
Jeong, Yu-Jin [1 ,3 ]
Oh, Jun Gu [1 ]
Yoo, Hee Joon [1 ]
Yang, Jiyeon [2 ]
Kwon, Jung-il [2 ]
Lee, Kwang-Won [1 ]
机构
[1] Korea Univ, Coll Life Sci & Biotechnol, Dept Biotechnol, Seoul 02841, South Korea
[2] Korea Univ, Grad Sch, Dept Integrated Biomed & Life Sci, Seoul 02841, South Korea
[3] Andel Inst, Dept Metab & Nutr Programming, Grand Rapids, MI 49503 USA
关键词
2 '-fucosyllactose; gut health; infant formula; human milk oligosaccharides; INTESTINAL EPITHELIAL-CELLS; HUMAN-MILK OLIGOSACCHARIDES; CHAIN FATTY-ACIDS; NECROTIZING ENTEROCOLITIS; INFLAMMATORY RESPONSES; MICROBIOTA; INFANTS; PERMEABILITY; METABOLITES; ACTIVATION;
D O I
10.3168/jds.2024-25171
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
This study investigated the potential of 2 '-fucosyllactose (2 '-FL) and galacto-oligosaccharides (GOS) combinations as a novel and cost-effective substitute for human milk oligosaccharides (HMO) in promoting gut health and reducing inflammation. In vitro studies using caco-2 cells showed that 2 '-FL and GOS combinations (H1 = GOS:2 '-FL ratio of 1.8:1; H2 = GOS:2 '-FL ratio of 3.6:1) reduced LPS-induced inflammation by decreasing pro-inflammatory markers, whereas individual treatments had no significant effects. In a mouse model of dextran sulfate sodium (DSS)-induced colitis, combined 2 '-FL and GOS supplementation alleviated symptoms, improved gut permeability, and enhanced intestinal structure, with the GH1 group (H1 combo with DSS) being the most effective. 2 '-Fucosyllactose and GOS combinations also enhanced short-chain fatty acid production in infant fecal batch fermentation and mouse fecal analysis, with GH1 showing the most promising results. The GH1 supplementation altered gut microbiota in mice with DSS-induced colitis, promoting microbial diversity and a more balanced Firmicutes to Bacteroidota ratio. Infant formula products (IFP) containing 2 '-FL and GOS combinations (IFP2 = 174 mg of GOS and 95 mg of 2 '-FL per 14 g serving, 1.8:1 ratio; IFP3 = 174 mg of GOS and 48 mg of 2 '-FL per 14 g serving, 3.6:1 ratio) demonstrated gastrointestinal protective and anti-inflammatory properties in a co-culture model of caco-2 and THP-1 cells. These findings suggest that 2 '-FL and GOS combinations have potential applications in advanced infant formulas and supplements to promote gut health and reduce inflammation.
引用
收藏
页码:10203 / 10220
页数:18
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