Exosomes from Human Periodontal Ligament Stem Cells Promote Differentiation of Osteoblast-like Cells and Bone Healing in Rat Calvarial Bone

被引：3

作者：

Albougha, Mhd Safwan ^{[1
]}

Sugii, Hideki ^{[1
]}

Adachi, Orie ^{[1
]}

Mardini, Bara ^{[1
]}

Soeno, Serina ^{[1
]}

Hamano, Sayuri ^{[1
]}

Hasegawa, Daigaku ^{[2
]}

Yoshida, Shinichiro ^{[2
]}

Itoyama, Tomohiro ^{[1
]}

Obata, Junko ^{[1
]}

Maeda, Hidefumi ^{[1
,2
]}

机构：

[1] Kyushu Univ, Fac Dent Sci, Dept Endodontol & Operat Dent, 3-1-1 Maidashi,Higashi ku, Fukuoka 8128582, Japan

[2] Kyushu Univ Hosp, Dept Endodontol, 3-1-1 Maidashi,Higashi Ku, Fukuoka 8128582, Japan

来源：

BIOMOLECULES | 2024年 / 14卷 / 11期

基金：

日本学术振兴会;

关键词：

periodontal ligament stem cells; exosomes; osteoblast-like cells; bone healing; GINGIVAL TISSUE SOURCES; EXTRACELLULAR VESICLES; SYSTEMATIC ANALYSIS; GLOBAL BURDEN; 195; COUNTRIES; REGENERATION; REPAIR; PREVALENCE; DISABILITY; EXPRESSION;

D O I：

10.3390/biom14111455

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Deep caries and severe periodontitis cause bone resorption in periodontal tissue, and severe bone resorption leads to tooth loss. Periodontal ligament stem cells (PDLSCs) are important for the healing of defective periodontal tissue. It is increasingly understood that healing of periodontal tissue is mediated through the secretion of trophic factors, particularly exosomes. This study investigated the effects of exosomes from human PDLSCs (HPDLSCs-Exo) on human osteoblast-like cells in vitro and on the healing of rat calvarial bone defects in vivo. HPDLSCs-Exo were isolated and characterized by their particle shape, size (133 +/- 6.4 nm), and expression of surface markers (CD9, CD63, and CD81). In vitro results showed that HPDLSCs-Exo promoted the migration, mineralization, and expression of bone-related genes such as alkaline phosphatase (ALP), bone morphogenetic protein 2 (BMP2), osteocalcin (OCN), and osteopontin (OPN) in human osteoblast-like cells. Furthermore, in vivo results showed that more newly formed bone was observed in the HPDLSCs-Exo-treated group than in the non-treated group at the defect sites in rats. These results indicated that HPDLSCs-Exo could promote osteogenesis in vitro and in vivo, and this suggests that HPDLSCs-Exo may be an attractive treatment tool for bone healing in defective periodontal tissue.

引用

页数：15

共 67 条

[1] Decorin Promotes Osteoblastic Differentiation of Human Periodontal Ligament Stem Cells [J].