Decadal Evolution of KPC-related plasmids in Pseudomonas aeruginosa high-risk clone ST463 in Zhejiang, China

被引：0
作者：
Li, Yue [1 ,2 ,3 ]
Yang, Qing [4 ,5 ]
Chen, Minhua [1 ,6 ]
Cai, Heng [1 ,2 ,3 ]
Fang, Li [1 ,2 ,3 ]
Zhou, Junxin [1 ,2 ,3 ]
Weng, Rui [1 ,2 ,3 ]
Ni, Hanming [1 ,2 ,3 ]
Jiang, Yan [1 ,2 ,3 ]
Hua, Xiaoting [1 ,2 ,3 ]
Yu, Yunsong [1 ,2 ,3 ]
机构：
[1] Zhejiang Univ, Sir Run Run Shaw Hosp, Dept Infect Dis, Sch Med, Hangzhou, Peoples R China
[2] Zhejiang Univ, Sir Run Run Shaw Hosp, Reg Med Ctr, Sch Med,Natl Inst Resp Dis, Hangzhou, Peoples R China
[3] Key Lab Microbial Technol & Bioinformat Zhejiang P, Hangzhou, Peoples R China
[4] Zhejiang Univ, Affiliated Hosp 1, Sch Med, Dept Lab Med, Hangzhou, Peoples R China
[5] Zhejiang Univ, Affiliated Hosp 1, Natl Clin Res Ctr Infect Dis, State Key Lab Diag & Treatment Infect Dis,Sch Med,, Hangzhou, Peoples R China
[6] Zhejiang Prov Peoples Hosp, Emergency & Crit Care Ctr, Intens Care Unit, Hangzhou, Peoples R China
来源：
COMMUNICATIONS BIOLOGY | 2024年 / 7卷 / 01期
基金：
中国国家自然科学基金;
关键词：
COPY NUMBER;
D O I：
10.1038/s42003-024-07337-5
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Klebsiella pneumoniae carbapenemase-producing Pseudomonas aeruginosa (KPC-PA) isolates have quickly expanded in China, especially the high-risk clone ST463. We aimed to explore the evolution of KPC-related plasmids driving ST463 clone success. Whole-genome sequencing of 1258 clinical P. aeruginosa strains (2011-2020) identified 106 ST463-PA isolates, with a KPC prevalence of 90.6%. Early on (2011-2012), ST463-PA obtained the KPC-encoding type II (pT2-KPC) or type I plasmid (pT1-KPC) to overcome carbapenem stress. Between 2012 and 2017, pT1-KPC plasmid dominated due to its lower fitness costs and IS26-driven blaKPC amplification ability. By 2017-2020, large fragment deletions in pT1-KPC formed pT1del-KPC plasmid. It conferred even lower fitness costs, enhanced blaKPC-2 gene stability, and greater copy-number flexibility, while maintaining horizontal transmission ability. Consequently, pT1del-KPC plasmid finally succeeded, making ST463 the dominant ST in China. Our findings highlight evolutionary pressures driving ST463 dominance and emphasize the need for targeted strategies to control its spread and antibiotic resistance development.
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页数：12
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