Association of Genes TRH, PRL and PRLR with Milk Performance, Reproductive Traits and Heat Stress Response in Dairy Cattle

被引:0
作者
Fang, Qianhai [1 ]
Zhang, Hailiang [1 ]
Gao, Qing [1 ]
Hu, Lirong [1 ]
Zhang, Fan [1 ]
Xu, Qing [2 ]
Wang, Yachun [1 ]
机构
[1] China Agr Univ, Coll Anim Sci & Technol, Natl Engn Lab Anim Breeding, Key Lab Anim Genet Breeding & Reprod,Minist Agr &, Beijing 100193, Peoples R China
[2] Beijing Jiaotong Univ, Inst Life Sci & Bioengn, Beijing 100044, Peoples R China
关键词
milk performance; fertility; heat stress; prolactin; prolactin receptor; thyrotropin-releasing hormone; THYROTROPIN-RELEASING-HORMONE; PROLACTIN RECEPTOR; CONCEPTION RATE; HOLSTEIN COWS; DRY PERIOD; POLYMORPHISMS; TEMPERATURE; PARAMETERS; SCORE; COLD;
D O I
10.3390/ijms26051963
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In our previous study, we found that changes in plasma prolactin (PRL) concentration were significantly associated with heat stress in dairy cows, and that PRL plays an important role in milk performance. Microarray sequencing revealed that thyrotropin releasing hormone (TRH) and prolactin receptor (PRLR), two genes important for PRL expression or function, may affect milk performance, reproduction, and heat stress response in dairy cattle. In this study, we further validated the genetic effects of the three genes in Chinese Holsteins. The potential variants within the three genes were first detected in 70 Chinese Holstein bulls and then screened in 1152 Chinese Holstein cows using the KASP (Kompetitive allele-specific PCR) method. In total, 42 variants were identified. Further, 13 SNPs were retained for KASP genotyping, including 8 in TRH, 3 in PRL, and 2 in PRLR. Using SNP-based association analyses, the multiple significant (p < 0.05) associations of these 13 SNPs with milk performance, reproduction, and heat stress response traits were found in the Holstein population. Furthermore, linkage disequilibrium analysis found a haplotype block in each of the TRH and PRL genes. Haplotype-based association analyses showed that haplotype blocks were also significantly (p < 0.05) associated with milk performance, reproduction, and heat stress response traits. Collectively, our results identified the genetic associations of TRH, PRL, and PRLR with milk performance, reproduction, and heat stress response traits in dairy cows, and found the important roles of SNP g.55888602A/C and g.55885455A/G in TRH in all traits, providing important molecular markers for genetic selection of high-yielding dairy cows.
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页数:16
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共 44 条
  • [1] Genome-wide association analysis in domestic animals: a powerful approach for genetic dissection of trait loci
    Andersson, Leif
    [J]. GENETICA, 2009, 136 (02) : 341 - 349
  • [2] Effects of photoperiod during the dry period on prolactin, prolactin receptor, and milk production of dairy cows
    Auchtung, TL
    Rius, AG
    Kendall, PE
    McFadden, TB
    Dahl, GE
    [J]. JOURNAL OF DAIRY SCIENCE, 2005, 88 (01) : 121 - 127
  • [3] Reproductive role of prolactin
    Bachelot, Anne
    Binart, Nadine
    [J]. REPRODUCTION, 2007, 133 (02) : 361 - 369
  • [4] Haploview: analysis and visualization of LD and haplotype maps
    Barrett, JC
    Fry, B
    Maller, J
    Daly, MJ
    [J]. BIOINFORMATICS, 2005, 21 (02) : 263 - 265
  • [5] The association of five polymorphisms with milk production traits in Czech Fleckvieh cattle
    Boleckova, J.
    Matejickova, J.
    Stipkova, M.
    Kyselova, J.
    Barton, L.
    [J]. CZECH JOURNAL OF ANIMAL SCIENCE, 2012, 57 (02) : 45 - 53
  • [6] Prolactin and Autoimmunity
    Borba, Vania Vieira
    Zandman-Goddard, Gisele
    Shoenfeld, Yehuda
    [J]. FRONTIERS IN IMMUNOLOGY, 2018, 9
  • [7] CABELL SB, 1990, J ANIM SCI, V68, P4292
  • [8] Genetic and Genomic Analyses of Service Sire Effect on Female Reproductive Traits in Holstein Cattle
    Chen, Ziwei
    Brito, Luiz F.
    Luo, Hanpeng
    Shi, Rui
    Chang, Yao
    Liu, Lin
    Guo, Gang
    Wang, Yachun
    [J]. FRONTIERS IN GENETICS, 2021, 12
  • [9] Remarkable genetic diversity detected at river buffalo prolactin receptor (PRLR) gene and association studies with milk fatty acid composition
    Cosenza, G.
    Iannaccone, M.
    Auzino, B.
    Macciotta, N. P. P.
    Kovitvadhi, A.
    Nicolae, I
    Pauciullo, A.
    [J]. ANIMAL GENETICS, 2018, 49 (03) : 159 - 168
  • [10] Ensembl 2019
    Cunningham, Fiona
    Achuthan, Premanand
    Akanni, Wasiu
    Allen, James
    Amode, M. Ridwan
    Armean, Irina M.
    Bennett, Ruth
    Bhai, Jyothish
    Billis, Konstantinos
    Boddu, Sanjay
    Cummins, Carla
    Davidson, Claire
    Dodiya, Kamalkumar Jayantilal
    Gall, Astrid
    Giron, Carlos Garcia
    Gil, Laurent
    Grego, Tiago
    Haggerty, Leanne
    Haskell, Erin
    Hourlier, Thibaut
    Izuogu, Osagie G.
    Janacek, Sophie H.
    Juettemann, Thomas
    Kay, Mike
    Laird, Matthew R.
    Lavidas, Ilias
    Liu, Zhicheng
    Loveland, Jane E.
    Marugan, Jose C.
    Maurel, Thomas
    McMahon, Aoife C.
    Moore, Benjamin
    Morales, Joannella
    Mudge, Jonathan M.
    Nuhn, Michael
    Ogeh, Denye
    Parker, Anne
    Parton, Andrew
    Patricio, Mateus
    Salam, Ahamed Imran Abdul
    Schmitt, Bianca M.
    Schuilenburg, Helen
    Sheppard, Dan
    Sparrow, Helen
    Stapleton, Eloise
    Szuba, Marek
    Taylor, Kieron
    Threadgold, Glen
    Thormann, Anja
    Vullo, Alessandro
    [J]. NUCLEIC ACIDS RESEARCH, 2019, 47 (D1) : D745 - D751