Cross-Sectional Assessment on Carbapenem-Resistant Gram-Negative Bacteria Isolated from Patients in Moldova

被引：0

作者：

Nirca, Vadim ^{[1
]}

Fuchs, Frieder ^{[2
,3
,4
,5
]}

Burgwinkel, Tessa ^{[5
,6
]}

del Pino, Rocio Arazo ^{[5
,6
]}

Zaharcenco, Ecaterina ^{[1
]}

Hagen, Ralf Matthias ^{[2
,3
]}

Poppert, Sven ^{[7
]}

Frickmann, Hagen ^{[8
,9
,10
]}

Higgins, Paul G. ^{[5
,6
]}

机构：

[1] Imunotehnomed Ltd, Mol Biol Dept, Kishinev MD-2001, Moldova

[2] Bundeswehr Cent Hosp Koblenz, Dept Microbiol, D-56070 Koblenz, Germany

[3] Bundeswehr Cent Hosp Koblenz, Hosp Hyg, D-56070 Koblenz, Germany

[4] Landesuntersuchungsamt Rheinland Pfalz, D-56068 Koblenz, Germany

[5] Univ Cologne, Univ Hosp Cologne, Inst Med Microbiol Immunol & Hyg, Fac Med, D-50931 Cologne, Germany

[6] German Ctr Infect Res DZIF, Partner Site Bonn Cologne, D-50935 Cologne, Germany

[7] Bernhard Nocht Inst Trop Med Hamburg, D-20359 Hamburg, Germany

[8] Bundeswehr Hosp Hamburg, Dept Microbiol, D-20359 Hamburg, Germany

[9] Bundeswehr Hosp Hamburg, Hosp Hyg, D-20359 Hamburg, Germany

[10] Univ Med Rostock, Inst Microbiol Virol & Hyg, D-18057 Rostock, Germany

来源：

MICROORGANISMS | 2025年 / 13卷 / 02期

关键词：

epidemiology; carbapenem-resistance; Gram-negative bacteria; surveillance; UKRAINE; MANAGEMENT;

D O I：

10.3390/microorganisms13020421

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Information on the molecular epidemiology and carbapenem resistance mechanisms in Gram-negative bacterial isolates in Moldova is scarce. To close this knowledge gap, carbapenem-resistant Gram-negative bacteria were collected over an 11-month period in a routine diagnostic laboratory in Moldova. Antimicrobial susceptibility was phenotypically and genotypically assessed. Phylogenetic relationships were investigated and multi-locus sequence types were provided. The assessment indicated several clusters of phylogenetically closely related carbapenem-resistant Klebsiella pneumoniae (sequence types ST101, ST395 and ST377), Acinetobacter baumannii (ST2, ST19 and ST78) and Pseudomonas aeruginosa (ST357 and ST654) isolates next to a number of less frequently observed species and sequence types. A phylogenetic relationship to characterized isolates from neighboring Ukraine could be confirmed. Identified carbapenemase genes comprised blaOXA-23, blaOXA-72 and blaGES-11 in A. baumannii, blaKPC-3, blaNDM-1 and blaOXA-48 in K. pneumoniae, as well as blaVIM-2 in Pseudomonas aeruginosa. In conclusion, the assessment suggested the spread of carbapenem-resistant Gram-negative bacteria in Moldova which were partly pre-described from neighboring Ukraine, as well as likely spill-over events, facilitating the regional spread of carbapenem-resistant clones. Several isolates with very high genomic similarity further support the hypothesis of likely regional transmission events driven by several evolutionary successful clonal lineages.

引用

页数：19

共 31 条

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