Formulation and evaluation of PVA-based composite hydrogels: physicochemical, leachables, and in vitro immunogenicity studies

被引：4

作者：

Himawan, Achmad ^{[1
,2
]}

Korelidou, Anna ^{[1
]}

Perez-Moreno, Ana M. ^{[3
,7
]}

Paris, Juan L. ^{[3
]}

Dominguez-Robles, Juan ^{[4
]}

Vora, Lalitkumar K. ^{[1
]}

Permana, Andi Dian ^{[2
]}

Larraneta, Eneko ^{[1
]}

Graham, Robert ^{[5
]}

Scott, Christopher J. ^{[6
]}

Donnelly, Ryan F. ^{[1
]}

机构：

[1] Queens Univ Belfast, Sch Pharm, Belfast BT9 7AF, North Ireland

[2] Univ Hasanuddin, Fac Pharm, Dept Pharmaceut Sci & Technol, Makassar 90245, Indonesia

[3] Inst Invest Biomed Malaga & Plataforma Nanomed IBI, Allergy Res Grp, Malaga 29590, Spain

[4] Univ Seville, Fac Pharm, Dept Pharm & Pharmaceut Technol, Seville 41004, Spain

[5] Queens Univ Belfast, Sch Biol Sci, Belfast BT9 7AF, North Ireland

[6] Queens Univ Belfast, Patrick G Johnson Ctr Canc Res, Belfast BT9 7BL, North Ireland

[7] Univ Malaga, Malaga 29071, Spain

来源：

JOURNAL OF MATERIALS CHEMISTRY B | 2025年 / 13卷 / 07期

基金：

欧盟地平线“2020”;

关键词：

FORMING MICRONEEDLE ARRAYS; CO-MALEIC ACID); SOLID-STATE NMR; POLY(VINYL ALCOHOL); DENDRITIC CELLS; POLYVINYL-ALCOHOL; DRUG-RELEASE; OXALIC-ACID; C-13; NMR; CHITOSAN;

D O I：

10.1039/d4tb02181a

中图分类号：

TB3 [工程材料学]; R318.08 [生物材料学];

学科分类号：

0805 ; 080501 ; 080502 ;

摘要：

This study explores the formulation and characterization of poly(vinyl alcohol) (PVA)-based composite hydrogels synthesized through solid-state crosslinking. Comprehensive assessments were conducted on their physicochemical properties, leachables, and immunogenicity. Swelling experiments demonstrated that the incorporation of poly(vinylpyrrolidone) (PVP) enhanced water retention, while chitosan had a minimal effect on swelling behavior. Qualitative analysis of leachables identified water-soluble components, including dehydrated PVA and PVP. Fourier-transform infrared (FTIR) spectroscopy confirmed the formation of ester bonds and indicated increased hydrogen bonding post-crosslinking. Thermal stability was validated by differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA), with decomposition observed at 320-330 degrees C. X-ray diffraction (XRD) analysis revealed enhanced crystallinity following crosslinking. Solid-state nuclear magnetic resonance (NMR) further confirmed chemical changes consistent with the results from other characterization techniques. In vitro assays using DC2.4 mouse dendritic cells showed that hydrogel extracts inhibited cell proliferation without causing cytotoxicity or triggering significant immune responses. These findings highlight the hydrogels' biocompatibility and stability, supporting their potential for biomedical applications.

引用

页码：2431 / 2445

页数：15

共 117 条

[1] Viscoelasticity, mechanical properties, andin vivobiocompatibility of injectable polyvinyl alcohol/bioactive glass composite hydrogels as potential bone tissue scaffolds [J].