Theileria annulata infects B-cells in sheep, which display lower dissemination potential compared to T. lestoquardi-infected ovine B-cells

被引：0

作者：

Tajeri, Shahin ^{[1
,2
,3
]}

de Late, Perle Latre ^{[2
,3
,5
]}

Hemmink, Johanneke D. ^{[4
,6
]}

Vrettou, Christina ^{[4
]}

Langsley, Gordon ^{[2
,3
]}

Morrison, W. Ivan ^{[4
]}

机构：

[1] Free Univ Berlin, Inst Parasitol & Trop Vet Med, D-14163 Berlin, Germany

[2] Inst Cochin, Inserm U1016 CNRS UMR8104, Paris, France

[3] Univ Paris Descartes Sorbonne Paris Cite, Fac Med, Lab Biol Comparat Apicomplexes, Paris, France

[4] Univ Edinburgh, Roslin Inst, Royal Dick Sch Vet Sci, Edinburgh EH25 9RG, Scotland

[5] Univ Missouri, Christopher S Bond Life Sci Ctr, 1201 Rollins St, Columbia, MO 65211 USA

[6] Int Livestock Res Inst ILRI, POB 30709, Nairobi, Kenya

来源：

TICKS AND TICK-BORNE DISEASES | 2025年 / 16卷 / 02期

关键词：

Theileria lestoquardi; Theileria annulata; Theileriosis; Leukocyte transformation; Sheep; IN-VITRO; MATRIX METALLOPROTEINASES; CROSS-IMMUNITY; UP-REGULATION; CATTLE; AP-1; TRANSCRIPTION; ATTENUATION; METASTASIS; EXPRESSION;

D O I：

10.1016/j.ttbdis.2025.102443

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Theileria annulata and Theileria lestoquardi are deadly tick-borne parasites of cattle and sheep, respectively. These parasites are transmitted by Hyalomma ticks, and their geographical distributions partially overlap, with T. annulata having a wider range. Theileria lestoquardi infection is highly pathogenic in its natural sheep and goat hosts while T. annulata infection usually causes a mild disease in these species. Interestingly, T. annulata does not produce merozoites/piroplasms in sheep and goats, therefore it is not tick-transmissible. The aim of the current study was to understand the basis of the attenuated pathogenicity of ovine infections by T. annulata. Theileria annulata and T. lestoquardi sporozoites were used to infect sheep and parasitized ovine leukocytes were isolated and phenotyped. This revealed that T. annulata sporozites target ovine B-cells, whereas T. lestoquardi sporozoite infection was not restricted to ovine B-cells. The ability of ovine B-cells infected with T. lestoquardi or T. annulata to traverse Matrigel in vitro was investigated, as a surrogate for their ability to disseminate in vivo and cause disease. The Matrigel traversal index ofT. lestoquardi-transformed ovine B-cells was significantly higher than that of T. annulata-transformed ovine B-cells isolated from the same host, consistent with the reported diminished pathogenicity of T. annulata infections in sheep. Theileria lestoquardi-transformed ovine B-cells preferentially expressed matrix metalloproteinase 2 (mmp2), whereas T. annulata-infected ovine B-cells strongly expressed mmp9. Correspondingly, MMP9 protein levels and collagenase activity were higher in T. annulata-transformed ovine B-cells. However, T. annulata-transformed ovine B-cells expressed higher levels of transcripts for Tissue Inhibitor of Metallopeptidases 1 and 2 (TIMP1 and TIMP2). TIMPs are the natural endogenous inhibitors of MMPs. This argues that their heightened expression could underpin the significantly lower Matrigel traversal activity of T. annulata-infected compared to T. lestoquardi-infected ovine B-cells.

引用

页数：8

共 47 条

[1] Adamson R., Hall R., A role for matrix metalloproteinases in the pathology and attenuation of Theileria annulata infections, Parasitol. Today, 13, 10, pp. 390-393, (1997)
[2] Adamson R., Logan M., Kinnaird J., Langsley G., Hall R., Loss of matrix metalloproteinase 9 activity in Theileria annulata-attenuated cells is at the transcriptional level and is associated with differentially expressed AP-1 species, Mol. Biochem. Parasitol., 106, 1, pp. 51-61, (2000)
[3] Adamson R.E., Hall F.R., Matrix metalloproteinases mediate the metastatic phenotype of Theileria annulata-transformed cells, Parasitology, 113, pp. 449-455, (1996)
[4] Akhter W., Aslam A., Rehman M.U., Rehman H.U., Rashid I., Akhtar R., Comparative pathological changes in sheep infected with Theileria annulata and non-infected control, J. Hellenic Vet. Med. Soc., 68, 4, pp. 629-634, (2018)
[5] Al-Fahdi A., Alqamashoui B., Al-Hamidhi S., Kose O., Tageldin M.H., Bobade P., Johnson E.H., Hussain A.R., Karagenc T., Tait A., Shiels B., Bilgic H.B., Babiker H., Molecular surveillance of Theileria parasites of livestock in Oman, Tick. Tick Borne Dis., 8, 5, pp. 741-748, (2017)
[6] Al-Hamidhi S., Weir W., Kinnaird J., Tageledin M., Beja-Pereira A., Morrison I., Thompson J., Tait A., Shiels B., Babiker H.A., Theileria lestoquardi displays reduced genetic diversity relative to sympatric Theileria annulata in Oman, Infect. Genet. Evol., 43, pp. 297-306, (2016)
[7] Albini A., Iwamoto Y., Kleinman H.K., Martin G.R., Aaronson S.A., Kozlowski J.M., McEwan R.N., A rapid in vitro assay for quantitating the invasive potential of tumor cells, Cancer Res, 47, 12, pp. 3239-3245, (1987)
[8] Ali A.M., Beyer D., Bakheit M.A., Kullmann B., Salih D.A., Ahmed J.S., Seitzer U., Influence of subculturing on gene expression in a Theileria lestoquardi-infected cell line, Vaccine, 26, 6, pp. G17-G23, (2008)
[9] Baylis H.A., Megson A., Brown C.G., Wilkie G.F., Hall R., Theileria annulata-infected cells produce abundant proteases whose activity is reduced by long-term cell culture, Parasitology, 105, pp. 417-423, (1992)
[10] Baylis H.A., Megson A., Hall R., Infection with Theileria annulata induces expression of matrix metalloproteinase 9 and transcription factor AP-1 in bovine leucocytes, Mol. Biochem. Parasitol., 69, 2, pp. 211-222, (1995)

← 1 2 3 4 5 →