Application of Mesenchymal Stem Cell-Derived Schwann Cell-like Cells Spared Neuromuscular Junctions and Enhanced Functional Recovery After Peripheral Nerve Injury

被引:0
作者
Nam, Yu Hwa [1 ]
Kim, Ji-Sup [2 ]
Yum, Yoonji [1 ]
Yoon, Juhee [1 ]
Song, Hyeryung [1 ]
Kim, Ho-Jin [3 ]
Lim, Jaeseung [3 ]
Park, Saeyoung [1 ]
Jung, Sung-Chul [1 ,4 ]
机构
[1] Ewha Womans Univ, Coll Med, Dept Biochem, Seoul 07804, South Korea
[2] Ewha Womans Univ, Seoul Hosp, Coll Med, Dept Orthoped Surg, Seoul 07804, South Korea
[3] Cellatoz Therapeut Inc, Seongnam 13487, South Korea
[4] Ewha Womans Univ, Grad Program Syst Hlth Sci & Engn, Seoul 07804, South Korea
关键词
tonsil-derived mesenchymal stem cells; Schwann cell-like cells; neuronal regeneration-promoting cells; peripheral nerve injury; nerve transection; neurorrhaphy; fibrin glue; nerve regeneration; neuromuscular junction; STROMAL CELLS; FIBRIN GLUE; REGENERATION; REPAIR; MICE;
D O I
10.3390/cells13242137
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
In general, the nerve cells of the peripheral nervous system regenerate normally within a certain period after the physical damage of their axon. However, when peripheral nerves are transected by trauma or tissue extraction for cancer treatment, spontaneous nerve regeneration cannot occur. Therefore, it is necessary to perform microsurgery to connect the transected nerve directly or insert a nerve conduit to connect it. In this study, we applied human tonsillar mesenchymal stem cell (TMSC)-derived Schwann cell-like cells (TMSC-SCs) to facilitate nerve regeneration and prevent muscle atrophy after neurorrhaphy. The TMSC-SCs were manufactured in a good manufacturing practice facility and termed neuronal regeneration-promoting cells (NRPCs). A rat model of peripheral nerve injury (PNI) was generated and a mixture of NRPCs and fibrin glue was transplanted into the injured nerve after neurorrhaphy. The application of NRPCs and fibrin glue led to the efficient induction of sciatic nerve regeneration, with the sparing of gastrocnemius muscles and neuromuscular junctions. This sparing effect of NRPCs toward neuromuscular junctions might prevent muscle atrophy after neurorrhaphy. These results suggest that a mixture of NRPCs and fibrin glue may be a therapeutic candidate to enable peripheral nerve and muscle regeneration in the context of neurorrhaphy in patients with PNI.
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页数:20
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