ANALYSIS OF PUTATIVE EgBBM PROMOTER BINDING PROTEINS DURING OIL PALM SOMATIC EMBRYOGENESIS

被引:0
作者
Khianchaikhan, Kamolwan [1 ]
Aroonluk, Suvichark [2 ]
Vuttipongchaikij, Supachai [1 ,3 ]
Jantasuriyarat, Chatchawan [1 ,3 ]
机构
[1] Kasetsart Univ, Fac Sci, Dept Genet, Bangkok 10900, Thailand
[2] Chandrakasem Rajabhat Univ, Fac Agr & Life Sci, Program Agr, Bangkok 10900, Thailand
[3] Kasetsart Univ, Natl Res Univ Kasetsart, Ctr Adv Studies Trop Nat Resources, CASTNAR,NRU KU, Bangkok 10900, Thailand
来源
JOURNAL OF OIL PALM RESEARCH | 2025年 / 37卷 / 01期
关键词
BABY BOOM (BBM); DNA-protein pull-down assay; somatic embryogenesis; SYSTEMIC ACQUIRED-RESISTANCE; ARMADILLO-RELATED PROTEINS; STEM-CELL MAINTENANCE; TRANSCRIPTION FACTOR; SEED-GERMINATION; ARABIDOPSIS SNI1; GENE-EXPRESSION; DNA-BINDING; RESPONSES; HETERODIMERIZATION;
D O I
10.21894/jopr.2023.0062
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
BABY BOOM (BBM) gene has been identified as a crucial factor in regulating somatic embryogenesis in plants. During this process, high levels of BBM expression are observed. Nonetheless, the mechanisms regulating the expression of BBM during somatic embryogenesis in oil palm remain unclear. In this study, we cloned and examined the cis-regulatory elements of the EgBBM promoter. Our analysis revealed the presence of cis-regulatory elements, such as ABRE, as-1, G-box, Sp1, STRE, ARE, CAT-Box and MYB, which are similar to those found in the promoters of AtBBM and OsBBM1. In addition, we conducted a DNA-protein pull-down assay followed by mass spectrometry to identify the potential binding proteins of the EgBBM promoter. Several proteins were related to histone modification through direct and indirect interaction for controlling the EgBBM expression. Therefore, this study highlighted a complex regulatory network formation of histone modification and putative EgBBM binding proteins through both DNA-protein and protein-protein interactions. Our findings provide valuable insights into the factors that influence the role of EgBBM in oil palm tissue culture during somatic embryogenesis.
引用
收藏
页码:72 / 84
页数:192
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