Erythrocytic α-Synuclein in Parkinson's Disease and Progressive Supranuclear Palsy-A Pilot Study

被引:3
作者
Cristiani, Costanza Maria [1 ]
Scaramuzzino, Luana [1 ]
Parrotta, Elvira Immacolata [2 ]
Cuda, Giovanni [3 ]
Quattrone, Aldo [1 ]
Quattrone, Andrea [1 ,4 ]
机构
[1] Magna Graecia Univ Catanzaro, Neurosci Res Ctr, Dept Med & Surg Sci, I-88100 Catanzaro, Italy
[2] Magna Graecia Univ Catanzaro, Inst Mol Biol, Dept Med & Surg Sci, I-88100 Catanzaro, Italy
[3] Magna Graecia Univ Catanzaro, Res Ctr Adv Biochem & Mol Biol, Dept Clin & Expt Med, I-88100 Catanzaro, Italy
[4] Magna Graecia Univ Catanzaro, Inst Neurol, Dept Med & Surg Sci, I-88100 Catanzaro, Italy
关键词
Parkinson's disease; progressive supranuclear palsy; alpha-synuclein; oligomeric alpha-synuclein; phosphorylated alpha-synuclein; red blood cells; RED-BLOOD-CELLS; POTENTIAL BIOMARKER; CEREBROSPINAL-FLUID; OLIGOMERS;
D O I
10.3390/biomedicines12112510
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Background/Objectives: The current research examines the accuracy of alpha-synuclein in RBCs as a diagnostic biomarker for PD and PSP, despite their distinct molecular etiologies. Methods: We used ELISA to measure total, oligomeric, and p129-alpha-synuclein levels in erythrocytes from 8 PSP patients, 19 PD patients, and 18 healthy controls (HCs). The classification performances of RBC alpha-synuclein levels were investigated by receiver operator characteristic (ROC) curve. We also evaluated a possible correlation between RBC alpha-synuclein level and the biological and clinical features of our cohorts. Results: RBC total alpha-synuclein was higher in PSP patients compared to both PD patients and HCs, achieving good classification performance (AUC: 0.853) in distinguishing PSP patients from PD patients, with a sensitivity of 100% and a specificity of 70.6%; moreover, the levels of this biomarker positively correlated with disease severity in PSP group. Regarding oligomeric alpha-synuclein and p129-alpha-synuclein, the latter was slightly increased in RBCs from PSP patients compared to HCs, but no correlations were detected. Conclusions: Although these findings need to be confirmed in larger studies, our pilot work suggests that RBC total alpha-synuclein may represent a potential molecular biomarker for the differential diagnosis and clinical staging of PSP.
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页数:10
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