Escherichia coli phage-inducible chromosomal island aids helper phage replication and represses the locus of enterocyte effacement pathogenicity island

被引:0
作者
Pick, Kat [1 ]
Stadel, Lauren [1 ]
Raivio, Tracy L. [1 ]
机构
[1] Univ Alberta, Dept Biol Sci, CW 405 Biol Sci Bldg, Edmonton, AB T6G 2E9, Canada
基金
加拿大健康研究院; 加拿大自然科学与工程研究理事会;
关键词
phage-inducible chromosomal island; mobile genetic elements; crosstalk; lysogeny; bacterial competition; virulence; excisionase; temperate bacteriophage; IMMUNE-SYSTEMS; HIGH-ACCURACY; PROPHAGES; GENOME; GENES; IDENTIFICATION; EXPRESSION; ALIGNMENT; PROTEINS; VECTORS;
D O I
10.1093/ismejo/wrae258
中图分类号
Q14 [生态学(生物生态学)];
学科分类号
071012 ; 0713 ;
摘要
In this study, we identify and characterize a novel phage-inducible chromosomal island (PICI) found in commensal Escherichia coli MP1. This novel element, EcCIMP1, is induced and mobilized by the temperate helper phage vB_EcoP_Kapi1. EcCIMP1 contributes to superinfection immunity against its helper phage, impacting bacterial competition outcomes. Genetic analysis of EcCIMP1 led us to uncover a putative transcriptional repressor, which silences virulence gene expression in the murine pathogen Citrobacter rodentium. We also found a putative excisionase encoded by EcCIMP1 which paradoxically does not promote excision of EcCIMP1 but rather supports excision of the helper phage. Another putative excisionase encoded by a presumed integrative conjugative element can also support the excision of vB_EcoP_Kapi1, demonstrating crosstalk between excisionases from multiple classes of mobile genetic elements within the same cell. Although phylogenetically distant from other characterized PICIs, EcCIMP1 and EcCIMP1-like elements are prevalent in both pathogenic and commensal isolates of E. coli from around the world, underscoring the importance of characterizing these abundant genetic elements.
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