Generation of TP53 knock out induced pluripotent stem cell using CRISPR/Cas9

被引：0

作者：

Zhou, Haonan ^{[1
,2
]}

Liu, Yaonan ^{[3
]}

Chen, Qu ^{[2
]}

Huang, Ke ^{[3
]}

Ren, Pei-Gen ^{[1
]}

机构：

[1] Chinese Acad Sci, Shenzhen Inst Adv Technol, Ctr Canc Immunol, Shenzhen 518055, Peoples R China

[2] Shenzhen Int Inst Biomed Res, Shenzhen 518116, Guangdong, Peoples R China

[3] Shenzhen Genocury Biotech CO LTD, Innovat Sq 1321,Pingshan Ave 2007,Pingshan St, Shenzhen 518118, Peoples R China

来源：

STEM CELL RESEARCH | 2025年 / 85卷

关键词：

TP53; iPSC; CRISPR/Cas9;

D O I：

10.1016/j.scr.2025.103699

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

The TP53 gene is an important tumor suppressor gene. Through CRISPR/Cas9 technology, we have established a TP53 gene knockout cell line in iPSCs (SIIBRi001-A). This cell line maintains normal stem cell-like morphology, karyotype, expresses markers of pluripotency, and is capable of generating teratomas in immunodeficient mice. Quantitative analysis of pluripotency gene expression remains normal. This cell line can be utilized for studying the mechanisms underlying tumorigenesis.

引用

页数：4