Electrochemical biosensor for point-of-care testing aqueous molecular hydrogen

Hydrogen (H2) is not only a crucial energy carrier but also a therapeutically significant gas with broad biological implications. Developing a sensitive, convenient, and reliable method for detecting and quantifying molecular hydrogen in aqueous solutions is essential to meet the growing demands of hydrogen medicine and health research. In this study, we present a portable hydrogenase-based electrochemical biosensor designed for detecting trace amounts of hydrogen in various aqueous environments. Our electrochemical biosensor offers highly sensitive detection of aqueous H2 within a linear range of 0-3 mu M, with a limit of detection (LOD) of 33 nM. The entire detection process is completed in just five minutes, and the sensor retains over 80 % of its activity even after six months of storage at 4 degrees C. This biosensor features exceptional sensitivity, stability, and robustness, making it scalable, adaptable, and portable. It is ideally suited for detecting a variety of beverages and clinical samples with low LOD and high reliability. These advancements pave the way for new commercial products in the hydrogen medicine and health industry and facilitate progress in developing diverse enzyme-based biosensors.