Magnetic Nanoparticle-Mediated Extraction and Electrochemical Detection of E. coli O157:H7 Genomic DNA

被引：0
作者：
Mendoza, David Joram [1 ]
Atienza-Parcon, Ma. Theresa Jonna A. [2 ,3 ]
Alocilja, Evangelyn C. [4 ]
Fernando, Lilia M. [5 ]
机构：
[1] Monash Univ, Dept Mat Sci & Engn, 20 Res Way, Clayton, Vic 3800, Australia
[2] Univ Philippines Banos, Natl Inst Mol Biol & Biotechnol, Nanobiotechnol Lab, Coll, Laguna, Philippines
[3] Southern Illinois Univ, Sch Agr Sci, Carbonadale, IL USA
[4] Michigan State Univ, Dept Biosyst & Agr Engn, Nanobiosensors Lab, E Lansing, MI USA
[5] Univ Philippines Banos Coll, Inst Crop Sci, Coll Agr & Food Sci, Biomat & Sensors Lab, 2-F AG Samonte Hall, Laguna 4031, Philippines
来源：
ANALYTICAL LETTERS | 2024年
关键词：
DNA-based nanobiosensor; E. coli O157:H7; magnetic nanoparticles; foodborne pathogen; stx1; gene; ESCHERICHIA-COLI; POLYANILINE NANOWIRE; O157-H7; BIOSENSORS; ELECTRODE; ACID;
D O I：
10.1080/00032719.2024.2425665
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
We report a rapid magnetic nanoparticle-mediated protocol to extract and detect DNA of E. coli O157:H7. Bare iron oxide nanoparticles (Fe3O4 NPs) were used as solid phase support to extract high purity DNA from concentrated and spiked water samples. Electrochemical detection of the DNA is achieved by employing polyaniline-functionalized magnetic nanoparticles (PANI-MNPs) which serve as DNA pre-concentrator and transducer. The DNA-based nanobiosensor shows good selectivity and sensitivity (detection limit of 0.10 ng/mu L DNA) and offers faster and comparable results relative to conventional methods. This DNA extraction and detection method is expected to be applicable in food, water, and environmental samples.
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页数：16
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共 39 条
  • [1] Allen J, 2001, Electrochemical methods: fundamentals and applications, V2nd
  • [2] PCR-less DNA co-polymerization detection of Shiga like toxin 1 (stx1) in Escherichia coli O157:H7
    Anderson, Michael J.
    Miller, Hanna R.
    Alocilja, Evangelyn C.
    [J]. BIOSENSORS & BIOELECTRONICS, 2013, 42 : 581 - 585
  • [3] Modified fractal iron oxide magnetic nanostructure: A novel and high performance platform for redox protein immobilization, direct electrochemistry and bioelectrocatalysis application
    Bagheri, Hasan
    Ranjbari, Elias
    Amiri-Aref, Mohaddeseh
    Hajian, Ali
    Ardakani, Yalda Hosseinzadeh
    Amidi, Salimeh
    [J]. BIOSENSORS & BIOELECTRONICS, 2016, 85 : 814 - 821
  • [4] Potential application of superparamagnetic nanoparticles for extraction of bacterial genomic DNA from contaminated food and environmental samples
    Basu, Semanti
    Chatterjee, Saptarshi
    Bandyopadhyay, Arghya
    Sarkar, Keka
    [J]. JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, 2013, 93 (04) : 788 - 793
  • [5] Comparison of methods for fluorescent detection of viable, dead, and total Escherichia coli O157:H7 cells in suspensions and on apples using confocal scanning laser microscopy following treatment with sanitizers
    Burnett, SL
    Beuchat, LR
    [J]. INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2002, 74 (1-2) : 37 - 45
  • [6] Reoccurring Escherichia coli O157:H7 Strain Linked to Leafy Greens-Associated Outbreaks, 2016-2019
    Chen, Jessica C.
    Patel, Kane
    Smith, Peyton A.
    Vidyaprakash, Eshaw
    Snyder, Caroline
    Tagg, Kaitlin A.
    Webb, Hattie E.
    Schroeder, Morgan N.
    Katz, Lee S.
    Rowe, Lori A.
    Howard, Dakota
    Griswold, Taylor
    Lindsey, Rebecca L.
    Carleton, Heather A.
    [J]. EMERGING INFECTIOUS DISEASES, 2023, 29 (09) : 1895 - 1899
  • [7] Strategies for the detection of Escherichia coli O157:H7 in foods
    Deisingh, AK
    Thompson, M
    [J]. JOURNAL OF APPLIED MICROBIOLOGY, 2004, 96 (03) : 419 - 429
  • [8] Recent advances in polyaniline based biosensors
    Dhand, Chetna
    Das, Maumita
    Datta, Monika
    Malhotra, B. D.
    [J]. BIOSENSORS & BIOELECTRONICS, 2011, 26 (06) : 2811 - 2821
  • [9] DNA-based electrochemical nanobiosensor for the detection of Phytophthora palmivora (Butler) Butler, causing black pod rot in cacao (Theobroma cacao L.) pods
    Franco, Anthony James D. M.
    Merca, Florinia E.
    Rodriguez, Myrna S.
    Balidion, Johnny F.
    Migo, Veronica P.
    Amalin, Divina M.
    Alocilja, Evangelyn C.
    Fernando, Lilia M.
    [J]. PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY, 2019, 107 : 14 - 20
  • [10] DETECTION OF ESCHERICHIA-COLI O157/H7 BY MULTIPLEX PCR
    FRATAMICO, PM
    SACKITEY, SK
    WIEDMANN, M
    DENG, MY
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1995, 33 (08) : 2188 - 2191
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