Magnetic Nanoparticle-Mediated Extraction and Electrochemical Detection of E. coli O157:H7 Genomic DNA

被引：0

作者：

Mendoza, David Joram ^{[1
]}

Atienza-Parcon, Ma. Theresa Jonna A. ^{[2
,3
]}

Alocilja, Evangelyn C. ^{[4
]}

Fernando, Lilia M. ^{[5
]}

机构：

[1] Monash Univ, Dept Mat Sci & Engn, 20 Res Way, Clayton, Vic 3800, Australia

[2] Univ Philippines Banos, Natl Inst Mol Biol & Biotechnol, Nanobiotechnol Lab, Coll, Laguna, Philippines

[3] Southern Illinois Univ, Sch Agr Sci, Carbonadale, IL USA

[4] Michigan State Univ, Dept Biosyst & Agr Engn, Nanobiosensors Lab, E Lansing, MI USA

[5] Univ Philippines Banos Coll, Inst Crop Sci, Coll Agr & Food Sci, Biomat & Sensors Lab, 2-F AG Samonte Hall, Laguna 4031, Philippines

来源：

ANALYTICAL LETTERS | 2025年 / 58卷 / 15期

关键词：

DNA-based nanobiosensor; E. coli O157:H7; magnetic nanoparticles; foodborne pathogen; stx1; gene; ESCHERICHIA-COLI; POLYANILINE NANOWIRE; O157-H7; BIOSENSORS; ELECTRODE; ACID;

D O I：

10.1080/00032719.2024.2425665

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

We report a rapid magnetic nanoparticle-mediated protocol to extract and detect DNA of E. coli O157:H7. Bare iron oxide nanoparticles (Fe3O4 NPs) were used as solid phase support to extract high purity DNA from concentrated and spiked water samples. Electrochemical detection of the DNA is achieved by employing polyaniline-functionalized magnetic nanoparticles (PANI-MNPs) which serve as DNA pre-concentrator and transducer. The DNA-based nanobiosensor shows good selectivity and sensitivity (detection limit of 0.10 ng/mu L DNA) and offers faster and comparable results relative to conventional methods. This DNA extraction and detection method is expected to be applicable in food, water, and environmental samples.

引用

页码：2593 / 2608

页数：16

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