Extraction and characterization of collagen and gelatin from body wall of sea cucumbers Stichopus horrens and Holothuria arenicola

被引:0
作者
Barzkar, Noora [1 ,2 ]
Attaran-Fariman, Gilan
Taheri, Ali [3 ]
Maran, Balu Alagar Venmathi [4 ]
机构
[1] Univ Malaysia Sabah, Higher Inst Ctr Excellence, Borneo Marine Res Inst, Kota Kinabalu, Sabah, Malaysia
[2] Chabahar Maritime Univ, Fac Marine Sci, Dept Marine Biol, Chabahar, Iran
[3] Chabahar Maritime Univ, Fac Marine Sci, Fisheries Dept, Chabahar, Iran
[4] Nagasaki Univ, Inst Integrated Sci & Technol, Nagasaki, Japan
关键词
Academic editor; Pepsin-solubilized collagen; Gelatin; Sea cucumber; Stichopus horrens; Holothuria arenicola; PEPSIN-SOLUBILIZED COLLAGEN; ACID-SOLUBLE COLLAGEN; PROXIMATE COMPOSITION; CHEMICAL-COMPOSITION; MINERAL CONTENTS; SKIN; FISH; ORIGIN; BONE; PURIFICATION;
D O I
暂无
中图分类号
TP18 [人工智能理论];
学科分类号
081104 ; 0812 ; 0835 ; 1405 ;
摘要
Background: Marine invertebrates, including sponges, molluscs, jellyfish, mussels, and sea cucumbers, are abundant sources of high-quality collagen and offer advantages such as availability, ease of processing, lower inflammatory response, and good metabolic compatibility. Approximately 70% of the total protein in the body wall of sea cucumbers is collagen. Gelatin is a water-soluble protein produced from heat-denatured collagen and has various industrial applications. Methods: Pepsin-solubilized collagen was extracted from the body wall of two sea cucumber Stichopus horrens and Holothuria arenicola, species found in the Oman Sea and characterized with SDS-PAGE and amino acid composition. Then gelatin was extracted from pepsin-solubilized collagen of S. horrens and some rheological properties were measured. Results: Amino acid composition and SDS-PAGE analysis showed that the collagen from both species was type I, with one a1 chain and beta chains, with molecular weights of 125 and 250 kDa, respectively. Glycine was the most abundant amino acid in the collagen from both sea cucumber species. The pepsin-soluble collagens from both species had high levels of glycine, proline, alanine, glutamic acid, and hydroxyproline. The gelatin from S. horrens had a melting point of 30 degrees C and displayed exceptional thermal stability, surpassing that of mammalian gelatin. Its gelling point was 5 degrees C, like that of cold-water fish gelatin, with a viscosity of 2.065 cp-lower than mammal gelatins. These findings suggested that collagen and gelatin from sea cucumbers could be useful in nutraceutical, pharmaceutical and cosmetic industries.
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页数:23
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