Isolation and Characterization of a Novel Escherichia Bacteriophage with Potential to Control Multidrug-Resistant Avian Pathogenic Escherichia coli and Biofilms

被引:0
作者
Wintachai, Phitchayapak [1 ,2 ,3 ]
Thaion, Fahsai [1 ,2 ]
Clokie, Martha R. J. [4 ]
Thomrongsuwannakij, Thotsapol [5 ,6 ]
机构
[1] Walailak Univ, Bacteriophage Lab, Nakhon Si Thammarat 80161, Thailand
[2] Walailak Univ, Sch Sci, Nakhon Si Thammarat 80161, Thailand
[3] Walailak Univ, Funct Mat & Nanotechnol Ctr Excellence, Nakhon Si Thammarat 80161, Thailand
[4] Univ Leicester, Dept Genet & Genome Biol, Leicester LE1 7RH, England
[5] Walailak Univ, Akkhraratchakumari Vet Coll, Nakhon Si Thammarat 80161, Thailand
[6] Walailak Univ, Ctr One Hlth, Nakhon Si Thammarat 80161, Thailand
来源
ANTIBIOTICS-BASEL | 2024年 / 13卷 / 11期
关键词
antibacterial activity; avian pathogenic Escherichia coli; bacteriophage; biofilms; phage therapy;
D O I
10.3390/antibiotics13111083
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
Background/Objectives: Avian pathogenic Escherichia coli (APEC) infection is a significant problem for the global chicken industry, as it decreases animal welfare and is associated with substantial economic losses. Traditionally, APEC infections have been controlled through the use of antibiotics, which has led to an increased prevalence of antibiotic-resistant E. coli. Therefore, developing alternative treatments for APEC infection is crucial. Methods: In this study, an Escherichia phage specific to multidrug-resistant (MDR) APEC, designated as phage vB_EcoP_PW8 (phage vECPW8), was isolated. The morphology, phage adsorption to host cells, one-step growth curve, thermal stability, pH stability, whole-genome sequencing, antibacterial ability, and antibiofilm efficacy of phage vECPW8 were evaluated. Results: The results demonstrated that phage vECPW8 has a Podoviridae morphology and is effective at lysing bacteria. Phage vECPW8 exhibited a high absorption rate to bacterial cells (more than 85% within 10 min) and had a latent period of 20 min, with a burst size of 143 plaque-forming units per cell. Additionally, phage vECPW8 showed good temperature and pH stability. The phage displayed strong antibacterial activity in vitro, and its efficacy in controlling bacteria was confirmed through scanning electron microscopy. Whole-genome sequencing revealed that the phage has a linear genome with 69,579 base pairs. The genome analysis supported the safety of the phage, as no toxin, virulence, or resistance-related genes were detected. Phage vECPW8 was identified as a novel lytic phage in the Gamaleyavirus genus and Schitoviridae family. The phage also demonstrated antibiofilm efficacy by reducing and preventing biofilm formation, as evidenced by biofilm biomass and bacterial cell viability measurements. Conclusions: These results indicate that phage vECPW8 is a promising candidate for the effective treatment of MDR APEC infections in poultry.
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页数:19
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  • [1] Bacteriophage Adsorption: Likelihood of Virion Encounter with Bacteria and Other Factors Affecting Rates
    Abedon, Stephen Tobias
    [J]. ANTIBIOTICS-BASEL, 2023, 12 (04):
  • [2] How to Name and Classify Your Phage: An Informal Guide
    Adriaenssens, Evelien M.
    Brister, J. Rodney
    [J]. VIRUSES-BASEL, 2017, 9 (04):
  • [3] Isolation, Molecular Characterization, and Antibiotic Resistance of Avian Pathogenic Escherichia coli in Eastern China
    Afayibo, Dosseh Jean Apotre
    Zhu, Hong
    Zhang, Beibei
    Yao, Lan
    Abdelgawad, Hosny Ahmed
    Tian, Mingxing
    Qi, Jingjing
    Liu, Yali
    Wang, Shaohui
    [J]. VETERINARY SCIENCES, 2022, 9 (07)
  • [4] Characterization, genome analysis and antibiofilm efficacy of lytic Proteus phages RP6 and RP7 isolated from university hospital sewage
    Ali, Sahd
    Karaynir, Abdulkerim
    Salih, Hanife
    Oncu, Serkan
    Bozdogan, Buelent
    [J]. VIRUS RESEARCH, 2023, 326
  • [5] Antimicrobial resistance of avian pathogenic Escherichia coli isolated from broiler, layer, and breeder chickens
    Bhattarai, Rebanta K.
    Basnet, Hom B.
    Dhakal, Ishwari P.
    Devkota, Bhuminand
    [J]. VETERINARY WORLD, 2024, 17 (02) : 480 - 499
  • [6] The habits of highly effective phages: population dynamics as a framework for identifying therapeutic phages
    Bull, James J.
    Gill, Jason J.
    [J]. FRONTIERS IN MICROBIOLOGY, 2014, 5
  • [7] Species prevalence, virulence genes, and antibiotic resistance of enterococci from food-producing animals at a slaughterhouse in Turkey
    Cebeci, Tugba
    [J]. SCIENTIFIC REPORTS, 2024, 14 (01):
  • [8] Physical Approaches to Prevent and Treat Bacterial Biofilm
    Ciarolla, Alexa A.
    Lapin, Norman
    Williams, Dustin
    Chopra, Rajiv
    Greenberg, David E.
    [J]. ANTIBIOTICS-BASEL, 2023, 12 (01):
  • [9] Bacteriophage therapy as an alternative biocontrol against emerging multidrug resistant E. coli in broilers
    Eid, Samah
    Tolba, Hala M. N.
    Hamed, Rehab, I
    Al-Atfeehy, Nayera M.
    [J]. SAUDI JOURNAL OF BIOLOGICAL SCIENCES, 2022, 29 (05) : 3380 - 3389
  • [10] Avian pathogenic, uropathogenic, and newborn meningitis-causing Escherichia coli:: How closely related are they?
    Ewers, Christa
    Li, Ganwu
    Wilking, Hendrik
    Kiessling, Sabine
    Alt, Katja
    Antao, Esther-Maria
    Laturnus, Claudia
    Diehl, Ines
    Glodde, Susanne
    Homeier, Timo
    Boehnke, Ute
    Steinrueck, Hartmut
    Philipp, Hans-C.
    Wieler, Lothar H.
    [J]. INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY, 2007, 297 (03) : 163 - 176