Bulge-Derived Epithelial Cells Isolated from Human Hair Follicles Using Enzymatic Digestion or Explants Result in Comparable Tissue-Engineered Skin

被引：0
作者：
Cattier, Bettina [1 ,2 ,3 ]
Guignard, Rina [1 ,2 ,3 ]
Martel, Israel [1 ,2 ,3 ]
Martel, Christian [1 ,2 ,3 ]
Simard-Bisson, Carolyne [1 ,2 ,3 ]
Larouche, Danielle [1 ,2 ,3 ]
Guiraud, Beatrice [4 ]
Bessou-Touya, Sandrine [4 ]
Germain, Lucie [1 ,2 ,3 ]
机构：
[1] Univ Laval, LOEX, Ctr Rech Organogenese Expt, Quebec City, PQ G1J 5B3, Canada
[2] Univ Laval, Fac Med, Dept Surg, Quebec City, PQ G1V 0A6, Canada
[3] Laval Univ, CHU Quebec, Res Ctr, Quebec City, PQ G1J 5B3, Canada
[4] Pierre Fabre Dermo Cosmet, R&D Ctr, F-31100 Toulouse, France
来源：
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2025年 / 26卷 / 05期
基金：
加拿大健康研究院;
关键词：
hair follicle; stem cells; regenerative medicine; tissue-engineered skin; cell culture technique; primary cell culture; FOLLICULAR STEM-CELLS; IN-VIVO; SERIAL CULTIVATION; KERATINOCYTES; GROWTH; MOUSE; DIFFERENTIATE; POPULATIONS; LOCATION;
D O I：
10.3390/ijms26051852
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Hair follicle stem cells, located in the bulge region of the outer root sheath, are multipotent epithelial stem cells capable of differentiating into epidermal, sebaceous gland, and hair shaft cells. Efficient culturing of these cells is crucial for advancements in dermatology, regenerative medicine, and skin model development. This investigation aimed to develop a protocol for isolating enriched bulge-derived epithelial cells from scalp specimens to produce tissue-engineered substitutes. The epithelium, including hair follicles, was separated from the dermis using thermolysin, followed by microdissection of the bulge region. Epithelial stem cells were isolated using enzymatic dissociation to create a single-cell suspension and compared with the direct explant culture and a benchmark method which isolates cells from the epidermis and pilosebaceous units. After 8 days of culture, the enzymatic digestion of microdissected bulges yielded 5.3 times more epithelial cells compared to explant cultures and proliferated faster than the benchmark method. Cells cultured from all methods exhibited comparable morphology and growth rates. The fully stratified epidermis of tissue-engineered skin was similar, indicating comparable differentiation potential. This enzymatic digestion method improved early-stage cell recovery and expansion while maintaining keratinocyte functionality, offering an efficient hair bulge cell-extraction technique for tissue engineering and regenerative medicine applications.
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页数：18
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