Advances and challenges in liquid chromatography-spectrometry (LC-MS) methodology for quantifying androgens and estrogens in human serum and plasma

被引:0
作者
Wang, Qingqing [1 ]
Mesaros, Clementina [1 ]
机构
[1] Univ Penn, Ctr Excellence Environm Toxicol, Dept Syst Pharmacol & Translat Therapeut, 856 BRB II-III 421 Curie Blvd, Philadelphia, PA 19104 USA
基金
美国国家卫生研究院;
关键词
Androgens; Estrogens; Liquid chromatography; Mass spectrometry; Derivatization; Chromatographic separation; Steroid hormones bioanalysis; TANDEM MASS-SPECTROMETRY; ASSAY REPRODUCIBILITY; POSTMENOPAUSAL WOMEN; FREE TESTOSTERONE; CORTISOL-LEVELS; QUANTIFICATION; ESTRADIOL; DERIVATIZATION; SPECIFICITY; SENSITIVITY;
D O I
10.1016/j.jsbmb.2024.106618
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Accurate quantification of androgens and estrogens is critical for elucidating their roles in endocrine disorders and advancing research on their functions in human biology and pathophysiology. This review highlights recent advances and ongoing challenges in liquid chromatography- mass spectrometry (LC- MS) methodology for quantifying androgens and estrogens in human serum and plasma. We summarized current approaches for analyzing the different forms of androgens and estrogens, along with their reported levels in publications from 2010 to the present. These published levels pointed out the inconsistencies in reference intervals across studies. To address these issues, advances in derivatization methods and chromatographic separation techniques are reviewed. Future perspectives for improving the accuracy and consistency of hormone quantification in clinical and research settings were also proposed.
引用
收藏
页数:14
相关论文
共 68 条
[1]   Separation of steroid isomers by ion mobility mass spectrometry [J].
Ahonen, Linda ;
Fasciotti, Maira ;
af Gennas, Gustav Boije ;
Kotiaho, Tapio ;
Daroda, Romeu J. ;
Eberlin, Marcos ;
Kostiainen, Risto .
JOURNAL OF CHROMATOGRAPHY A, 2013, 1310 :133-137
[2]   Parallel targeted and non-targeted quantitative analysis of steroids in human serum and peritoneal fluid by liquid chromatography high-resolution mass spectrometry [J].
Andrieu, Thomas ;
du Toit, Therina ;
Vogt, Bruno ;
Mueller, Michael D. ;
Groessl, Michael .
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2022, 414 (25) :7461-7472
[3]   New quantification method for estradiol in the prostatic tissues of benign prostatic hyperplasia using liquid chromatography-tandem mass spectrometry [J].
Arai, Seiji ;
Miyashiro, Yoshimichi ;
Shibata, Yasuhiro ;
Kashiwagi, Bunzo ;
Tomaru, Yukio ;
Kobayashi, Mikio ;
Watanabe, Yoko ;
Honma, Seijiro ;
Suzuki, Kazuhiro .
STEROIDS, 2010, 75 (01) :13-19
[4]   Reference intervals of nine steroid hormones over the life-span analyzed by LC-MS/MS: Effect of age, gender, puberty, and oral contraceptives [J].
Bae, Yoon Ju ;
Zeidler, Robert ;
Baber, Ronny ;
Vogel, Mandy ;
Wirkner, Kerstin ;
Loeffler, Markus ;
Ceglarek, Uta ;
Kiess, Wieland ;
Koerner, Antje ;
Thiery, Joachim ;
Kratzsch, Juergen .
JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY, 2019, 193
[5]   Radioimmunoassay and tandem mass spectrometry measurement of bedtime salivary cortisol levels: A comparison of assays to establish hypercortisolism [J].
Baid, Smita K. ;
Sinaii, Ninet ;
Wade, Matt ;
Rubino, Domenica ;
Nieman, Lynnette K. .
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 2007, 92 (08) :3102-3107
[7]  
Baldini V, 2005, J Endocrinol Invest, V28, P69
[8]   New Approach for the Identification of Isobaric and Isomeric Metabolites [J].
Ben Faleh, Ahmed ;
Warnke, Stephan ;
Van Wieringen, Teun ;
Abikhodr, Ali H. ;
Rizzo, Thomas R. .
ANALYTICAL CHEMISTRY, 2023, 95 (18) :7118-7126
[9]   Analysis of estrogens in serum and plasma from postmenopausal women: Past present, and future [J].
Blair, Ian A. .
STEROIDS, 2010, 75 (4-5) :297-306
[10]   Direct measurement of serum free testosterone by ultrafiltration followed by liquid chromatography tandem mass spectrometry [J].
Chen, Yu ;
Yazdanpanah, Mehrdad ;
Wang, Xiao Yan ;
Hoffman, Barry R. ;
Diamandis, Eleftherios P. ;
Wong, Pui-Yuen .
CLINICAL BIOCHEMISTRY, 2010, 43 (4-5) :490-496