Single-cell RNA sequencing reveals the heterogeneity of myofibroblasts in wound repair

被引：0

作者：

Liu, Miaonan ^{[1
]}

Liu, Xiaoxuan ^{[1
]}

Zhang, Jingchi ^{[1
]}

Liang, Shaocong ^{[1
]}

Gong, Yan ^{[2
]}

Shi, Shengjun ^{[2
]}

Yuan, Xiaopeng ^{[1
,3
,4
]}

机构：

[1] Southern Med Univ, Zhujiang Hosp, Dept Lab Med, Guangzhou, Peoples R China

[2] Southern Med Univ, Zhujiang Hosp, Dept Burns & Wound Repairing, Guangzhou, Peoples R China

[3] Southern Univ Sci & Technol, Shenzhen Peoples Hosp, Affiliated Hosp 1, Dept Lab Med, Shenzhen 518020, Guangdong, Peoples R China

[4] Jinan Univ, Clin Med Coll 2, Shenzhen 518020, Guangdong, Peoples R China

来源：

GENOMICS | 2025年 / 117卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Single-cell RNA sequencing; Myofibroblast; Heterogeneity; Skin ulcer; Keloid; MATRIX METALLOPROTEINASES; MOLECULAR-BASIS; EXPRESSION; KELOIDS; FIBROBLAST; MECHANISMS; MANAGEMENT; PROTEIN;

D O I：

10.1016/j.ygeno.2024.110982

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Skin wound repair involves myofibroblasts crucial for tissue integrity. This study utilized single-cell RNA sequencing to explore myofibroblast diversity in various wound healing scenarios. Analysis of 89,148 cells from skin ulcers, keloids, and normal scars identified 13 cell clusters. Myofibroblast subcluster analysis unveiled 11 subsets, with subclusters 1 and 9 predominant in ulcers. Subcluster 1 exhibited heightened matrix metalloproteinase expression and involvement in bacterial response and angiogenesis, crucial in inflammation. Tissue validation confirmed subcluster 1 significance., while animal models supported upregulated CA12, TDO2, and IL- 7R in chronic ulcers. These findings illuminate myofibroblast heterogeneity and their impact on wound healing, offering insights into potential therapeutic targets.

引用

页数：16

共 55 条

[1] The Role of Podoplanin in Skin Diseases [J].

Asai, Jun .

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2022, 23 (03)

[2] Metalloproteinase inhibitors: biological actions and therapeutic opportunities [J].

Baker, AH ;

Edwards, DR ;

Murphy, G .

JOURNAL OF CELL SCIENCE, 2002, 115 (19) :3719-3727

[3] Studies of transforming growth factors beta 1-3 and their receptors I and II in fibroblast of keloids and hypertrophic scars [J].

Bock, O ;

Yu, HY ;

Zitron, S ;

Bayat, A ;

Ferguson, MWJ ;

Mrowietz, U .

ACTA DERMATO-VENEREOLOGICA, 2005, 85 (03) :216-220

[4] Periostin action in bone [J].

Bonnet, Nicolas ;

Garnero, Patrick ;

Ferrari, Serge .

MOLECULAR AND CELLULAR ENDOCRINOLOGY, 2016, 432 (0C) :85-92

[5] Keloids: Current concepts of pathogenesis (Review) [J].

Bran, Gregor M. ;

Goessler, Ulrich R. ;

Hormann, Karl ;

Riedel, Frank ;

Sadick, Haneen .

INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, 2009, 24 (03) :283-293

[6] Single-Cell RNA-seq Analysis Reveals Cellular Functional Heterogeneity in Dermis Between Fibrotic and Regenerative Wound Healing Fates [J].

Chen, Cao-Jie ;

Kajita, Hiroki ;

Takaya, Kento ;

Aramaki-Hattori, Noriko ;

Sakai, Shigeki ;

Asou, Toru ;

Kishi, Kazuo .

FRONTIERS IN IMMUNOLOGY, 2022, 13

[7] Matrix Metalloproteinases: From Molecular Mechanisms to Physiology, Pathophysiology, and Pharmacology [J].

de Almeida, Luiz G. N. ;

Thode, Hayley ;

Eslambolchi, Yekta ;

Chopra, Sameeksha ;

Young, Daniel ;

Gill, Sean ;

Devel, Laurent ;

Dufour, Antoine .

PHARMACOLOGICAL REVIEWS, 2022, 74 (03) :712-768

[8] Single-cell RNA-seq reveals fibroblast heterogeneity and increased mesenchymal fibroblasts in human fibrotic skin diseases [J].

Deng, Cheng-Cheng ;

Hu, Yong-Fei ;

Zhu, Ding-Heng ;

Cheng, Qing ;

Gu, Jing-Jing ;

Feng, Qing-Lan ;

Zhang, Li-Xue ;

Xu, Ying-Ping ;

Wang, Dong ;

Rong, Zhili ;

Yang, Bin .

NATURE COMMUNICATIONS, 2021, 12 (01)

[9] Smooth muscle-derived adventitial progenitor cells direct atherosclerotic plaque composition complexity in a Klf4-dependent manner [J].

Dubner, Allison M. ;

Lu, Sizhao ;

Jolly, Austin J. ;

Strand, Keith A. ;

Mutryn, Marie F. ;

Hinthorn, Tyler ;

Noble, Tysen ;

Nemenoff, Raphael A. ;

Moulton, Karen S. ;

Majesky, Mark W. ;

Weiser-Evans, Mary C. M. .

JCI INSIGHT, 2023, 8 (22)

[10] Single-cell expression profiling reveals dynamic flux of cardiac stromal,vascular and immune cells in health and injury [J].

Farbehi, Nona ;

Patrick, Ralph ;

Dorison, Aude ;

Xaymardan, Munira ;

Janbandhu, Vaibhao ;

Wystub-Lis, Katharina ;

Ho, Joshua W. K. ;

Nordon, Robert E. ;

Harvey, Richard P. .

ELIFE, 2019, 8

← 1 2 3 4 5 6 →