Considerations for Domestication of Novel Strains of Filamentous Fungi

被引：1

作者：

Pullen, Randi M. ^{[1
]}

Decker, Stephen R. ^{[2
]}

Subramanian, Venkataramanan ^{[2
]}

Adler, Meaghan J. ^{[1
,3
]}

Tobias, Alexander V. ^{[1
,4
]}

Perisin, Matthew ^{[1
,5
]}

Sund, Christian J. ^{[1
]}

Servinsky, Matthew D. ^{[1
]}

Kozlowski, Mark T. ^{[1
]}

机构：

[1] DEVCOM Army Res Lab, Adelphi, MD 20783 USA

[2] Natl Renewable Energy Lab, Golden, CO 80401 USA

[3] Univ Tennessee, 1311 Cumberland Ave,307 Ken & Blaire Mossman Bldg, Knoxville, TN 37996 USA

[4] MITRE Corp, 7525 Colshire Dr, Mclean, VA 22102 USA

[5] Lallemand Inc, 67 Etna Rd 200, New Hampshire 03766, Lebanon

来源：

ACS SYNTHETIC BIOLOGY | 2025年 / 14卷 / 02期

关键词：

synthetic biology; filamentous fungi; molecularbiology; microbial cell factory; AGROBACTERIUM-MEDIATED TRANSFORMATION; BIOSYNTHETIC GENE CLUSTERS; ASPERGILLUS-NIDULANS; NEUROSPORA-CRASSA; FUNCTIONAL GENOMICS; TRICHODERMA-REESEI; HYPOCREA-JECORINA; EFFICIENT TRANSFORMATION; HOMOLOGOUS RECOMBINATION; HETEROLOGOUS EXPRESSION;

D O I：

10.1021/acssynbio.4c00672

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Fungi, especially filamentous fungi, are a relatively understudied, biotechnologically useful resource with incredible potential for commercial applications. These multicellular eukaryotic organisms have long been exploited for their natural production of useful commodity chemicals and proteins such as enzymes used in starch processing, detergents, food and feed production, pulping and paper making and biofuels production. The ability of filamentous fungi to use a wide range of feedstocks is another key advantage. As chassis organisms, filamentous fungi can express cellular machinery, and metabolic and signal transduction pathways from both prokaryotic and eukaryotic origins. Their genomes abound with novel genetic elements and metabolic processes that can be harnessed for biotechnology applications. Synthetic biology tools are becoming inexpensive, modular, and expansive while systems biology is beginning to provide the level of understanding required to design increasingly complex synthetic systems. This review covers the challenges of working in filamentous fungi and offers a perspective on the approaches needed to exploit fungi as microbial cell factories.

引用

页码：343 / 362

页数：20

共 283 条

[81] A novel carbon source-dependent genetic transformation system for the versatile cell factory Hypocrea jecorina (anamorph Trichoderma reesei) [J].