Schistosoma mansoni x S. haematobium hybrids frequently infecting sub-Saharan migrants in southeastern Europe: Egg DNA genotyping assessed by RD-PCR, sequencing and cloning

被引：0

作者：

De Elias-Escribano, Alejandra ^{[1
,2
]}

Artigas, Patricio ^{[1
,2
]}

Salas-Coronas, Joaquin ^{[2
,3
,4
]}

Luzon-Garcia, Maria Pilar ^{[3
,4
]}

Reguera-Gomez, Marta ^{[1
]}

Cabeza-Barrera, Maria Isabel ^{[3
]}

Vazquez-Villegas, Jose ^{[5
]}

Boissier, Jerome ^{[6
]}

Mas-Coma, Santiago ^{[1
,2
]}

Bargues, Maria Dolores ^{[1
,2
]}

机构：

[1] Univ Valencia, Fac Farm, Dept Parasitol, Burjassot 46100, Valencia, Spain

[2] Inst Salud Carlos III, Ctr Invest Biomed Red Enfermedades Infecciosas CIB, Madrid, Spain

[3] Hosp Univ Poniente, Trop Med Unit, El Ejido, Almeria, Spain

[4] Univ Almeria, Fac Hlth Sci, Dept Nursing Physiotherapy & Med, La Canada De San Urbano, Almeria, Spain

[5] Dist Sanitario Poniente Almeria, Trop Med Unit, El Ejido, Almeria, Spain

[6] Univ Montpellier, IFREMER Univ Perpignan Via Domitia, IHPE, CNRS, Perpignan, France

来源：

PLOS NEGLECTED TROPICAL DISEASES | 2025年 / 19卷 / 03期

关键词：

UROGENITAL SCHISTOSOMIASIS; CORSICA FRANCE; EPIDEMIOLOGY; TRANSMISSION; MORBIDITY; HYBRIDIZATIONS; EMPHASIS; CHILDREN; PARASITE; SENEGAL;

D O I：

10.1371/journal.pntd.0012942

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Background Globalization and neglected tropical diseases (NTDs) are increasingly closely linked. In recent years, Spain and Southern Europe are experiencing a considerable increase in the influx of migrants infected by NTDs, mainly from West African countries. This study focuses on imported schistosomiasis and the entry into Europe of hetero-specific hybrids between two human species, Schistosoma mansoni and S. haematobium, causing intestinal and urogenital schistosomiasis respectively.Methodology/principal findings Individualized genetic identification by molecular analysis using RD-PCR, sequencing and cloning of nuclear rDNA and mtDNA of 134 Schistosoma eggs was performed, including 41 lateral-spined and 84 terminal-spined eggs from urine, and nine lateral-spined eggs from stools. These eggs were recovered from six migrant males from Senegal, Guinea-Bissau, C & ocirc;te d'Ivoire and Mali, who shared ectopic shedding of S. mansoni-like eggs in their urine. A high hybridization complexity was detected in the eggs of these patients, involving three Schistosoma species. The six patients were infected by S. mansoni x S. haematobium hybrids shedding S. mansoni-like eggs, and also S. haematobium x S. curassoni hybrids shedding S. haematobium-like eggs. SmxSh hybrids were mostly detected in S. mansoni-like eggs from urine (94.59%), whereas in feces the detection of those hybrids was less frequent (5.41%).Conclusions/significance This study contributes to: (i) a better understanding of the heterospecific hybrids between S. mansoni and S. haematobium from the genetic point of view; (ii) it shows the frequency with which they are entering non-endemic countries, such as Spain and consequently in Europe; (iii) it determines the diversity of hybrid eggs and haplotypes that can occur within a single patient, e.g., up to two types of hybrids involving three Schistosoma species and up to six different haplotypes; (iv) it provides information to be considered in clinical presentations, diagnosis, responses to treatment and epidemiological impact in relation to possible transmission and establishment in non-endemic areas.

引用

页数：27