Antioxidant and renal protective effects of Nano-selenium on adenine-induced acute renal failure in canines

被引：1

作者：

Zhang, Mengdi ^{[1
,2
]}

Gao, Jindong ^{[1
]}

Kulyar, Md. F. ^{[2
]}

Luo, Wanhe ^{[1
,2
]}

Zhang, Guodong ^{[1
]}

Yang, Xiaoqi ^{[2
]}

Zhang, Tianguang ^{[2
]}

Gao, Haihang ^{[2
]}

Peng, Yuxuan ^{[2
]}

Zhang, Jiabin ^{[2
]}

Altaf, Muhammad ^{[3
]}

Algharib, Samah Attia ^{[4
]}

Zhou, Donghai ^{[2
]}

He, Jianzhong ^{[1
]}

机构：

[1] Tarim Univ, Coll Anim Sci, Engn Lab Tarim Anim Dis Diag & Control, Alar 843300, Xinjiang, Peoples R China

[2] Huazhong Agr Univ, Coll Vet Med, Wuhan 430070, Hubei, Peoples R China

[3] Islamia Univ Bahawalpur, Fac Vet & Anim Sci, Dept Clin Med & Surg, Bahawalpur 63100, Pakistan

[4] Benha Univ, Fac Vet Med, Dept Clin Pathol, Toukh 13736, QG, Egypt

来源：

ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY | 2024年 / 287卷

基金：

中国国家自然科学基金;

关键词：

Nano-selenium; dog; acute renal failure; keap1; oxidative stress; OXIDATIVE STRESS; NRF2; NANOPARTICLES; INJURY; DAMAGE; DOGS;

D O I：

10.1016/j.ecoenv.2024.117274

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Acute renal failure is a common clinical disease in canines, affecting antioxidant levels and decreasing the body's resistance. This study aims to explore the therapeutic mechanism of Nano-selenium in acute renal failure. The histopathological and imaging changes of kidney tissue were observed with the gene and protein expression levels of Keap1, Nrf2, HO-1, and NQO1 in the kidney. According to our findings, adding nano-selenium can effectively reduce the concentration of CRE and BUN in blood and kidney tissues. It increased the activity of GSHPX and SOD by an effective reduction of MDA. Through pathological and imaging observations, it was found that adding nano-selenium could improve the kidney tissue structure of acute renal failure. The results of the RTqPCR experiment showed that after the addition of nano-selenium, the mRNA expression of the Keap1 gene decreased significantly. In contrast, the mRNA expression of the Nrf2, HO-1, and NQO1 genes increased significantly. The experimental results were further verified by western blot and immunohistochemical analysis. Hence, the nano-selenium intervention improved kidney function and increased antioxidant levels in canines suffering from acute renal failure with the involvement of the Keap1-Nrf2/ARE signaling pathway.

引用

页数：9

共 39 条

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